Abstract: Objective: To investigate the anti-fatigue properties in mice of water extracts from nine different types of medicinal and edible component compositions, including Rubi fructus, Polygonati rhizoma, and Laminariae thallus Eckloniae Thallus. Methods: A group of 10 healthy KM mice, half female and half male, were randomly assigned to each of the following groups: blank control (equal volume of distilled water), positive control (Ginseng Royal Jelly Oral Liquid, 0.62 g/mL), and low, medium, and high (0.21, 0.62, 1.85 g/mL) dosage groups of water extract of compound Polygonati rhizoma composition. The mice were gavaged continuously for 30 days and the data were collected during gavage. At the end of the experiment, the mice's strenuous swimming time, organ index, and serum urea nitrogen, liver, and muscle glycogen contents were examined. Results: The water extract of the compound Polygonati rhizoma composition did not statistically differ from the blank control group in terms of the mice's weight (P>0.05), and no abnormalities were observed in the mice's organs (P>0.05). The water extract of compound Polygonati rhizoma composition has the potential to greatly increase mouse endurance, extend their strenuous swimming time (P<0.05), lower the amount of urea nitrogen in the water (P<0.01), and boost mouse efficiency (P<0.01). Conclusion: Exercise tiredness is mitigated by the water extract of the compound Polygonati rhizoma composition.