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中国精品科技期刊2020
徐博,姜汀,金旺德,等. 基于转录组测序探究OSW-1抗肝癌的作用机制[J]. 华体会体育,2024,45(24):1−8. doi: 10.13386/j.issn1002-0306.2023120158.
引用本文: 徐博,姜汀,金旺德,等. 基于转录组测序探究OSW-1抗肝癌的作用机制[J]. 华体会体育,2024,45(24):1−8. doi: 10.13386/j.issn1002-0306.2023120158.
XU Bo, JIANG Ting, JIN Wangde, et al. Evaluating the Anticancer Potential of Ornithogalum saundersiae Saponins on Hepatocellular Carcinoma: A Transcriptomic Approach[J]. Science and Technology of Food Industry, 2024, 45(24): 1−8. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120158.
Citation: XU Bo, JIANG Ting, JIN Wangde, et al. Evaluating the Anticancer Potential of Ornithogalum saundersiae Saponins on Hepatocellular Carcinoma: A Transcriptomic Approach[J]. Science and Technology of Food Industry, 2024, 45(24): 1−8. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120158.

基于转录组测序探究OSW-1抗肝癌的作用机制

Evaluating the Anticancer Potential of Ornithogalum saundersiae Saponins on Hepatocellular Carcinoma: A Transcriptomic Approach

  • 摘要: 目的:基于转录组测序发掘虎眼万年青皂苷(Ornithogalum saundersiae Saponins,OSW-1)作用于肝癌细胞(HEP3B)后的关键通路及差异表达基因。方法:利用转录组测序技术检测OSW-1处理HEP3B的基因表达数据,通过测定的序列信息精确地分析转录本,筛选与细胞存活相关的表达差异基因,得到核心基因。并通过实验验证OSW-1是否通过调控核心靶点治疗肝细胞癌。结果:MTT结果表明,经OSW-1给药后HEP3B细胞活性明显降低,且呈浓度依赖性。通过转录组测序检测,共获得1381个存在明显表达差异的基因,并且多数基因富集在TNF信号通路。进一步通过免疫荧光及Western blot表明,OSW-1干预HEP3B细胞后,p-PI3K、p-AKT、p-NF-κB蛋白水平表达降低。结论:OSW-1可能通过调控炎症信号通路降低炎症反应,抑制HEP3B细胞活性,可作为潜在的治疗肝癌的药物,以及为保肝护肝的保健食品研发提供实验依据。

     

    Abstract: Objective: To explore the crucial pathways and genes that are expressed differently when hepatocellular carcinoma cells (HEP3B) were treated with Ornithogalum saundersiae Saponins (OSW-1), using transcriptome sequencing. Methods: The gene expression data in HEP3B treated with OSW-1 were obtained through transcriptome sequencing technology. The transcripts were analyzed precisely based on the sequence information measured, and genes with differential expression related to cell survival were identified to discover the core genes. Through experimental methods, it was intended to confirm whether OSW-1 could treat hepatocellular carcinoma by targeting core genes. Results: The MTT assay revealed a significant reduction in HEP3B cell activity following OSW-1 treatment, which was dependent on the concentration. A total of 1381 genes showed significant differences in expression as a result of transcriptome sequencing, with a majority being involved in the TNF signaling pathway. Additionally, immunofluorescence and Western blot analysis indicated that the levels of p-PI3K, p-AKT, and p-NF-κB proteins were reduced in HEP3B cells after treatment with OSW-1. Conclusion: OSW-1 may suppress inflammation and reduce HEP3B cell activity by modulating inflammatory signaling pathways, suggesting its potential as a drug for liver cancer treatment and providing an experimental foundation for the development of healthy foods aimed at liver protection.

     

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