牡丹花酶解肽的理化性质及促进皮肤健康功效

曾贝贝; 吕静; 鲁杏茹; 智文博; 李带根

doi:10.13386/j.issn1002-0306.2023110278

牡丹花酶解肽的理化性质及促进皮肤健康功效

Physical and Chemical Properties of an Enzymolysis Peptide of Peony Flower and Beneficial Effect on Skin Health

摘要

摘要: 为进一步促进牡丹花的开发利用，本研究以酶解的牡丹花酶解肽为研究对象，分析了其分子量分布与氨基酸组成，通过测定体外酪氨酸酶抑制率、抗糖化活性、透明质酸酶抑制率以及抗氧化活性研究其促进皮肤健康的功效，并进一步利用斑马鱼黑色素沉积模型进行验证。结果表明，牡丹花酶解肽是由15种氨基酸组成的平均分子量为644 Da的小分子肽，当浓度为10 mg/mL时，体外酪氨酸酶抑制率为88.96%，晚期糖基化终末产物（AGEs）抑制率为90.50%，透明质酸酶抑制率为60.43%，DPPH自由基清除率和羟自由基清除率分别为74.77%和94.74%。同时，斑马鱼模型的结果表明牡丹花酶解肽可有效抑制斑马鱼头部黑色素沉积，当样品浓度为1000 μg/mL时，黑色素抑制率达到40.59%。总之，体内外研究结果初步证明牡丹花酶解肽具有良好的促进皮肤健康的潜力。

Abstract: The enzymolysis peptide of peony flower was studied herein to promote the development and utilization of this flower. The molecular weight distribution and amino acid composition of the peptide were analyzed, and the beneficial effects of the peptide were studied by assessing their ability to inhibit tyrosinase activity, advanced glycation end products (AGEs) formation, and hyaluronidase in vitro. The in vitro results were further verified in a zebrafish melanin accumulation model, results indicated that the active peptide was a small molecule comprising 15 amino acids, with an average molecular weight of 644 Da. At 10 mg/mL, the respective in vitro inhibition rates for tyrosinase activity, AGEs formation, and hyaluronidase were 88.96%, 90.50%, and 60.43%, respectively. The respective DPPH (2,2-diphenyl-1-picrylhydrazyl) and hydroxyl free radical scavenging rates were 74.77% and 94.74%, respectively. The zebrafish model studies revealed a melanin inhibition rate of 40.59% when the sample concentration was 1000 μg/mL. In conclusion, the results of these in vivo and in vitro studies preliminarily indicate that the peony flower enzymolysis peptide can potentially improve skin health.