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中国精品科技期刊2020
曾雨,杨晓迪,胡卓炎,等. 不同品种荔枝壳中原花青素的鉴定及其体外抗氧化活性研究[J]. 华体会体育,2024,45(21):218−226. doi: 10.13386/j.issn1002-0306.2023110041.
引用本文: 曾雨,杨晓迪,胡卓炎,等. 不同品种荔枝壳中原花青素的鉴定及其体外抗氧化活性研究[J]. 华体会体育,2024,45(21):218−226. doi: 10.13386/j.issn1002-0306.2023110041.
ZENG Yu, YANG Xiaodi, HU Zhuoyan, et al. Identification of Procyanidins in the Pericarps of Different Litchi Cultivars and Their in Vitro Antioxidant Activities[J]. Science and Technology of Food Industry, 2024, 45(21): 218−226. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110041.
Citation: ZENG Yu, YANG Xiaodi, HU Zhuoyan, et al. Identification of Procyanidins in the Pericarps of Different Litchi Cultivars and Their in Vitro Antioxidant Activities[J]. Science and Technology of Food Industry, 2024, 45(21): 218−226. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110041.

不同品种荔枝壳中原花青素的鉴定及其体外抗氧化活性研究

Identification of Procyanidins in the Pericarps of Different Litchi Cultivars and Their in Vitro Antioxidant Activities

  • 摘要: 为筛选适合开发天然抗氧化产品的荔枝优良品种,本研究对7个不同品种荔枝壳中总酚、总原花青素、总黄酮含量及A型低聚原花青素组分进行了分析,采用DPPH、ABTS自由基清除法及FRAP法测定其体外抗氧化活性。结果表明,褐毛荔果壳提取物中所含抗氧化活性成分如总酚、总原花青素、总黄酮以及总A型低聚原花青素含量均为最高。经电喷雾液质联用四级杆飞行时间质谱(HPLC-Q-TOF-MS/MS)分析鉴定,得到所含物质的一级及二级质谱。质谱结果显示荔枝壳中含有的低聚原花青素组分主要为表儿茶素、原花青素A2、原花青素A4、A型三聚体及四聚体,不同品种荔枝壳中原花青素含量具有差异性(P<0.05)。褐毛荔果壳提取物表现出较强的自由基清除活性(DPPH∙:1144.60 μmol Trolox/g DW,ABTS∙:2905.56 μmol Trolox/g DW,FRAP:868.84 μmol Trolox/g DW)。经皮尔逊相关性分析,原花青素单体、二聚体、三聚体含量与抗氧化活性具有显著相关性(P<0.05)。综合分析,与其他品种相比,褐毛荔果壳提取物中A型低聚原花青素含量最高,体外抗氧化活性方面表现最佳,可作为开发天然抗氧化产品的新型潜在原料加以利用。

     

    Abstract: To screen eminent litchi varieties suitable for the development of natural antioxidant products, the total phenol, total proanthocyanidin, total flavonoid contents and A-type oligomeric procyanidin fractions in the pericarps of seven diverse litchi cultivars were analyzed. The in vitro antioxidant activities including DPPH, ABTS+ free radical scavenging and FRAP were determined. The results demonstrated that the total phenols, total proanthocyanidins, total flavonoids as well as total A-type oligomeric procyanidins contained in the pericarp extract of 'Hemaoli' were the highest. In addition, the primary and secondary mass spectra of the contained substances were analyzed and identified by electrospray liquid-mass spectrometry with quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS/MS). The mass spectrometry results demonstrated that the oligomeric procyanidin fractions contained in litchi pericarps were mainly epicatechin, procyanidin A2, procyanidin A4, A-type trimer and tetramer. Moreover, the content of A-type oligomeric procyanidins in litchi pericarp varied significantly (P<0.05) among diverse cultivars. The pericarp extract of 'Hemaoli' possessed potent free radical scavenging activity (DPPH∙: 1144.60 μmol Trolox/g DW, ABTS+∙: 2905.56 μmol Trolox/g DW, FRAP: 868.84 μmol Trolox/g DW). The contents of A-type procyanidin monomer, dimer and trimer were significantly correlated with antioxidant activity by Pearson's correlation analysis (P<0.05). In summary, a comprehensive analysis indicated that the pericarp extract of 'Hemaoli' contained the highest content of A-type oligomeric procyanidins as well as remarkable antioxidant activity compared to other cultivars. Therefore, 'Hemaoli' could be utilized as a novel potential material for the development of natural antioxidant products.

     

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