酵母抽提物与姜黄素复合对急性酒精性肝损伤小鼠肠道菌群的调节作用

黄晓英; 余海立; 郑明山; 刘晓雯; 张彦

doi:10.13386/j.issn1002-0306.2023100198

酵母抽提物与姜黄素复合对急性酒精性肝损伤小鼠肠道菌群的调节作用

Regulation of Yeast Extract-Curcumin on Intestinal Flora in Mice with Acute Alcoholic Liver Injury

摘要

摘要: 目的：研究酵母抽提物、姜黄素+酵母抽提物复合物对小鼠急性酒精性肝损伤的预防保护作用及其对肠道菌群的调节作用，并探讨其保护作用机制。方法：建立急性酒精性肝损伤小鼠模型，分别给予酵母抽提物和姜黄素+酵母抽提物复合物，检测小鼠血清中谷丙转氨酶（Alanine aminotrans-ferase，ALT）、谷草转氨酶（Aspartate transaminase，AST）和肝组织中总谷胱甘肽（Toal glutathione，T-GSH）、谷胱甘肽过氧化物酶（Glutathione peroxidase，GSH-Px）、丙二醛（Malonicdialdehyde，MDA）、总胆固醇（Total cholesterol，TC）、甘油三酯（Triglyceride，TG）的变化，观察肝组织病理切片损伤情况，分析肠道菌群等指标。结果：与模型组相比，酵母抽提物、姜黄素+酵母抽提物复合物能够降低小鼠ALT、AST、MDA、TC和TG的水平，提高GSH-Px、T-GSH的水平；肝组织切片表明，受试物明显改善酒精引起的肝细胞脂肪浸润和炎症浸润；肠道菌群分析表明，酒精性肝损伤小鼠肠道菌群结构发生变化，姜黄素+酵母抽提物复合物能有效恢复因酒精改变的不动杆菌属、普雷沃菌属、瘤胃球菌属的相对丰度，效果优于酵母抽提物。结论：酵母抽提物、姜黄素+酵母抽提物复合物对小鼠急性酒精性肝损伤有明显的保护作用，其机制可能与调节氧化应激、脂质代谢、肠道菌群有关。

Abstract: Objective: This study aimed to investigate the protective effect and mechanism of yeast extract and curcumin-yeast extract on acute alcoholic liver injury in mice. Methods: The mouse model of acute alcoholic liver injury was established, and the mice were administered with yeast extract and curcumin-yeast extract. Changes in ALT, AST, T-GSH, GSH-Px, and MDA levels were observed in the serum and liver tissue, respectively. Pathological sections of liver tissue were examined to observe the damage. The intestinal flora of mice was analyzed. Results: Compared with the model group, both yeast extract and curcumin-yeast extract reduced the levels of ALT, AST, MDA, TC, and TG, increased the activities of GSH-Px and T-GSH, and alleviated fatty infiltration and inflammatory infiltration of hepatocytes. Curcumin-yeast extract effectively restored the abundance of Acinetobacter, Prevotella, and Ruminococcus, which had been altered by alcohol. The restorative effect was superior to that of the yeast extract group. Conclusion: Yeast extract and curcumin-yeast extract showed significant protective effects against acute alcoholic liver injury in mice. This mechanism may be associated with the regulation of oxidative stress, lipid metabolism, and intestinal flora.