黄芪多糖复合酶提取工艺优化及其<i>α</i>-葡萄糖苷酶抑制活性

张会佳; 侯相竹; 张涵; 殷澳; 高阳; 徐多多

doi:10.13386/j.issn1002-0306.2023090315

黄芪多糖复合酶提取工艺优化及其α-葡萄糖苷酶抑制活性

Optimization of Complex Enzyme Extraction Process of Astragalus Polysaccharides and Its α-Glucosidase Inhibitory Activities

摘要

摘要: 目的：以黄芪为原料，采用复合酶法（木瓜蛋白酶、果胶酶、纤维素酶）提取黄芪多糖（Astragalus polysaccharides，APS），并分析工艺条件对多糖提取的影响。方法：在正交试验确定复合酶比例的基础上，采用响应面法对复合酶提取APS的提取条件进行优化，得到最优工艺条件，采用pNPG法评价其α-葡萄糖苷酶抑制活性。结果：5 g黄芪药材粉末，最佳复合酶配比为：木瓜蛋白酶88000 U、果胶酶65000 U、纤维素酶6000 U；最佳酶解提取条件为：酶解处理时间、温度、pH和料液比分别为2.82 h、60.34 ℃、5.11和1:34.46 g/mL，APS的得率最高可达22.79%±0.14%；APS对α-葡萄糖苷酶的半数抑制浓度（IC₅₀）为7.42 μg/mL。结论：复合酶提取APS的得率较单酶得率显著提高，APS对α-葡萄糖苷酶表现出较强的抑制作用。

Abstract: Objective: To extract Astragalus polysaccharides (APS) from Astragalus membranaceus using compound enzymes (including papain, pectinase and cellulase), and to analyze the effect of process conditions on APS extraction. Methods: Orthogonal experiments were conducted to determine the proportion of compound enzymes. The response surface method was used to optimize the conditions of extracting APS with compound enzymes. The optimal process condition was obtained, and the α-glucosidase-inhibiting effect of APS was evaluated by pNPG method. Results: For 5 g of Astragalus herbs, the optimal ratio of papain, pectinase and cellulase were 88000 U, 65000 U and 6000 U, respectively. The optimal extraction condition was as follows: time of 2.82 h, temperature at 60.34 ℃, pH of 5.11, and solid-liquid ratio of 1:34.46 g/mL. The yield of APS under the optimal condition was up to 22.79%±0.14%. The half-inhibition concentration (IC₅₀) of APS for α-glucosidase was 7.42 μg/mL. Conclusion: The yield of APS by compound enzymes is significantly higher than that by single enzymes, and APS can effectively inhibit the activity of α-glucosidase.