大豆分离蛋白和大豆肽对鼠李糖乳杆菌GG生长代谢的影响

张银肖; 张弛; 王静怡; 温雁超; 李赫; 刘新旗

doi:10.13386/j.issn1002-0306.2023080237

大豆分离蛋白和大豆肽对鼠李糖乳杆菌GG生长代谢的影响

Effects of Soybean Protein Isolates and Soybean Peptides on Growth and Metabolism of Lactobacillus rhamnosus GG

摘要

摘要: 为探究氮源类营养物质对鼠李糖乳杆菌GG（Lactobacillus rhamnosus GG，LGG）生长和代谢的影响，本研究以模拟胃肠道消化后的大豆分离蛋白和大豆肽为原料，通过单培养的方式分别测定鼠李糖乳杆菌GG的活菌数和乳酸及乙酸产量，并将鼠李糖乳杆菌GG与单增李斯特菌共培养测定其在致病菌存在下利用大豆蛋白和大豆肽的情况。结果表明，在单培养的条件下消化后大豆分离蛋白和大豆肽都能显著提高鼠李糖乳杆菌的活菌数（P<0.05），而消化后大豆肽的作用比消化后大豆蛋白的作用提前4 h，且二者都能显著提高乳酸和乙酸的生成（P<0.05）。在共培养体系中，消化后大豆蛋白显著削弱了单增李斯特菌的竞争能力，提高了鼠李糖乳杆菌的竞争能力，且培养4 h和8 h后，鼠李糖乳杆菌的活细胞数显著高于单培养（P<0.05）。本研究结果将氮源作为具有潜在益生功能的营养物质，为消化后大豆蛋白和大豆肽功能性食品的开发提供理论依据。

Abstract: To investigate the effects of nitrogen source nutrients on the growth and metabolism of Lactobacillus rhamnosus GG (LGG), this study used soybean protein isolates (dSPI) and soybean peptides (dPEP) from simulated gastrointestinal digestion as raw materials. The number of viable cells and the production of lactic acid and acetic acid in LGG were measured through mono-culture. LGG was co-cultured with Listeria monocytogenes to determine its utilization of soybean protein isolates and soybean peptides in the presence of pathogens. The results of mono-culture showed that both dPEP and dSPI could increase the viable number of LGG (P<0.05), and the effect of dPEP was earlier four hours than that of dSPI, and they could significantly increase the production of SCFAs (P<0.05). In the co-culture system, dSPI weakened the competitiveness of Listeria monocytogenes (List m) and improved the competitiveness of LGG. After 4 h and 8 h of culture, the number of living cells of LGG was higher than that of mono-culture (P<0.05). The results of this study identify nitrogen sources as potentially beneficial nutrients, and provide a theoretical basis for the development of soybean protein and soybean peptide functional foods.