Abstract: Bacillus protease, a microbial protease known for its diverse range, significant activity and extensive research, has been widely used in the food industry. This review covers various aspects, including the screening and selection of protease high-producing Bacillus strains, the construction of engineered bacteria, the optimization of fermentation conditions for protease production, and the application of Bacillus protease in the food industry, specifically with plant proteins and animal proteins as substrates. Moreover, the future research direction and development trend are forecasted. The purpose of this review is to serve as a scholarly resource for future investigation and practical utilization of Bacillus protease.