黄芩提取物抗UVA诱导NIH3T3细胞光老化的作用

蔡春梅; 黄玉叶; 李丽燕; 余悦; 刘小娟; 肖树雄; 卢丽

doi:10.13386/j.issn1002-0306.2023070036

黄芩提取物抗UVA诱导NIH3T3细胞光老化的作用

Scutellaria baicalensis Extract Inhibits the UVA-induced Photoaging in NIH3T3 Cells

摘要

摘要: 目的：探究黄芩提取物对紫外线（UVA）诱导的成纤维细胞系（NIH3T3）光老化的保护作用。方法：通过UVA照射建立细胞光老化模型，采用CCK-8检测、β-半乳糖苷酶染色、DAPI染色、免疫荧光染色等技术，探讨黄芩提取物对光老化成纤维细胞的保护作用。结果：黄芩提取物能显著促进NIH3T3的细胞增殖与迁移（P<0.05），提高UVA照射后NIH3T3细胞存活率、降低UVA照射引起的细胞凋亡及衰老相关胞外β-半乳糖苷酶（SA-β-gal）的表达，并促进内源性I型、IV型胶原蛋白再生，逆转UVA照射引起的胶原蛋白流失，多方面共同作用抵御细胞光老化。结论：黄芩提取物能有效抑制因UVA照射引起的NIH3T3细胞光老化。

Abstract: Objective: To investigate the protective effect of Scutellaria baicalensis extract on ultraviolet (UVA)-induced photoaging in a fibroblast cell line (NIH3T3). Methods: The cellular photoaging model was established by UVA irradiation, and the protective effects of Scutellaria baicalensis extract on photoaging fibroblasts were investigated using CCK-8 assay, β-galactosidase staining, DAPI staining, and immunofluorescence staining. Results: Scutellaria baicalensis extract significantly promoted cell proliferation and migration of NIH3T3 cells (P<0.05), increased the cell viability of NIH3T3 cells after UVA irradiation, reduced UVA-induced cell apoptosis, and the expression of senescence-associated marker β-galactosidase (SA-β-gal). It also promoted the regeneration of endogenous type I and type IV collagen, reversing collagen loss induced by UVA irradiation, collectively contributing to the defense against cellular photoaging. Conclusion: Scutellaria baicalensis extract effectively inhibits the photoaging of NIH3T3 cells induced by UVA irradiation.