Abstract: In order to enhance the stability of anthocyanins and broaden its application in the fields of food health care and medicine, carboxymethyl chitosan (CMCS), L-histidine (L-His) and stearic acid (SA) were used as carrier materials, and Lonicera edulis pomace anthocyanins were used as core materials. The preparation process of acid-responsive Lonicera edulis pomace anthocyanins micelles was optimized by response surface method. The stability of Lonicera edulis pomace anthocyanins under different conditions such as light, temperature and pH and their cytotoxicity to liver cancer cells were evaluated. The results showed that the optimal preparation process was as follows: Water bath time 10 min, ultrasonic time 15 min, ultrasonic temperature 60 °C, and the encapsulation efficiency was 85.3%±0.31%. The storage stability analysis showed that under various conditions of temperature, pH, light, oxidant and reducing agent, the preservation rate of anthocyanins in the micelles were 1.33, 1.26, 1.16, 1.11 and 2.41 times that of anthocyanins, respectively. In the in vitro liver cancer cell killing experiment, after 24 h, the inhibition rate of anthocyanin-loaded micelles on liver cancer cells was 1.34 times that of anthocyanin. In this study, a novel amphiphilic carboxymethyl chitosan derivative was synthesized, which could significantly improve the stability of anthocyanins and was suitable as a potential nanomicelle carrier for anthocyanins and other food active ingredients.