Abstract: In this study, the lactic acid bacteria from the feces of Uygur mother-infant pairs in Hotan, Xinjiang, was isolated and identified. The bacteriostatic performance of the isolates for diarrhea pathogenic bacteria was assessed, and the probiotic properties were investigated in vitro. According to the groEL gene sequencing, 113 isolates of lactic acid bacteria representing 15 species were isolated from 19 mother-infant pairs, with Ligilactobacillus salivarius and Companilactobacillus farciminis being the dominant species, and 29 strains had inhibition effect on all the 6 pathogenic bacteria were screened from all strains. Among the strains with antibacterial activity, Companilactobacillus farciminis HTb36X-2 had the highest survival rate of 69.50% after being treated with simulated gastrointestinal juices for 4 h. Limosilactobacillus reuteri HTb34X5-6 and HTb34X5-1 had the highest auto-aggregation and hydrophobicity, with the hydrophobicity of 31.67% and 29.00%, and auto-aggregation of 81.32% and 70.91%, respectively. Combined with antibiotic resistance and carbohydrate metabolism of all 29 strains, a total of 7 strains of Companilactobacillus farciminis HTb36X-2, Lacticaseibacillus paracasei HTb6X-6, Lacticaseibacillus casei 17X-4, 17X-5, Limosilactobacillus reuteri HTb34X-5-1, HTb34X-5-6, Limosilactobacillus fermentum 10D12-2 were screened as potential probiotics, which might take a foundation for the development of probiotic and products with anti-diarrhea function.