玉米麸皮阿拉伯木聚糖复合纳米载体负载香草醛及其抑菌性能研究

安迪; 蔡翔; 吕雯欣; 陆雅琦; 李丹; 马福敏

doi:10.13386/j.issn1002-0306.2023030132

玉米麸皮阿拉伯木聚糖复合纳米载体负载香草醛及其抑菌性能研究

Study on Vanillin Loaded on Corn Bran Arabinoxylan Composite Nanocarrier and Its Antibacterial Property

摘要

摘要: 本研究构建了负载香草醛的玉米麸皮阿拉伯木聚糖（Arabinoxylan，AX）与玉米醇溶蛋白（Zein）复合纳米载体，通过多糖与蛋白质的相互作用稳定体系，提高香草醛的稳定性并探讨了复合纳米载体对单增李斯特菌和大肠杆菌的抑菌效果。用80%和90%乙醇沉淀得到两个玉米麸皮粗多糖组分，通过阴离子交换柱纯化后，得到四个组分（F80-1、F80-2、F90-1及F90-2），采用气相色谱法鉴定了各组分的单糖组成。结果表明，各组分阿拉伯糖和木糖总含量分别为67.41%、76.12%、77.16%和84.89%，四个组分复合纳米载体对香草醛的包封率分别达到了81.18%、69.36%、86.80%和88.48%，荧光光谱法和差示扫描量热法（DSC）验证了香草醛在复合纳米载体中的包埋效果及分子间相互作用，抑菌试验结果表明，F80-2制备的复合纳米载体抑制单增李斯特菌效果最好，F90-2复合纳米载体抑制大肠杆菌效果最好。

Abstract: In order to improve the stability of vanillin by polysaccharide-protein interaction and to investigate the inhibitory effect of the composite nanocarrier on Listeria monocytogenes and E. coli, a vanillin-loaded composite nanocarrier of corn bran arabinoxylan (AX) and zein was prepared. Two crude polysaccharide fractions of corn bran were obtained by precipitating with 80% and 90% ethanol. Four fractions (F80-1, F80-2, F90-1, and F90-2) were subjected to anion-exchange chromatography purification. The monosaccharide composition of the fractions was determined using gas chromatography. It was found that the total content of arabinose and xylose in each fraction was 67.41%, 76.12%, 77.16%, and 84.89%, respectively, and the encapsulation rate of vanillin in the composite nanoparticles of the four fractions was 81.18%, 69.36%, 86.80%, and 88.48%, respectively. The vanillin encapsulation and the intermolecular interaction in the composite nanocarriers were verified by fluorescence spectrometry and differential scanning calorimetry (DSC). Bacterial inhibition experiments showed that F80-2 composite nanocarriers were most effective at inhibiting Listeria monocytogenes, and F90-2 composite nanocarriers were most effective at inhibiting E. coli.