临沂和周口地区中高温大曲细菌群落结构与基因功能差异性研究

马梦月; 郭壮; 李学思; 管桂坤; 刘宇; 李富强; 王玉荣

doi:10.13386/j.issn1002-0306.2023030055

临沂和周口地区中高温大曲细菌群落结构与基因功能差异性研究

Research on the Differences in Bacteria Community Structure and Gene Function of Medium-High Temperature Daqu in Linyi and Zhoukou Areas

摘要

摘要: 为明确山东临沂和河南周口地区中高温大曲细菌群落结构及菌群基因功能的差异，本研究采用MiSeq高通量测序技术对2个地区中高温大曲的细菌群落结构进行了解析，并基于直系同源蛋白数据库（clusters of orthologous groups of proteins，COG）分析了其细菌类群潜在的功能。α多样性发现，两者在丰富度和多样性上差异均不显著（P>0.05）。相较于周口地区大曲，在门水平上，临沂地区大曲中放线菌门（Actinobacteria）和拟杆菌门（Bacteroidetes）平均相对丰度均显著偏低（P<0.05），在属水平上，不动杆菌属（Acinetobacter）平均相对丰度显著偏高（P<0.05），地杆菌属（Geobacter）和泛菌属（Pantoea）平均相对丰度均显著偏低（P<0.05）。LEfSe（linear discriminant analysis effect size）分析发现，Geobacter为周口地区大曲中的生物标志物，而临沂地区大曲中的生物标志物未在属水平体现；β多样性发现，两者在整体上存在明显差异；PICRUSt（phylogenetic investigation of communities by reconstruction of unobserved states）分析发现，临沂地区中高温大曲在转录、氨基酸和核苷转运与代谢功能上显著高于周口地区大曲（P<0.05）。由此可见，2个地区中高温大曲的细菌群落结构及菌群基因功能表达均存在明显差异。

Abstract: In order to clarify the differences in the community structure and gene function of medium-high temperature Daqu bacteria in Linyi, Shandong Province and Zhoukou, Henan Province. MiSeq high-throughput sequencing technology was used to analyze the bacterial community structure of medium-high temperature Daqu in the two regions, and the potential functions of bacterial taxa were analyzed based on the clusters of orthologous groups of proteins (COG). The result of α diversity showed that there were no significant differences in richness and diversity (P>0.05). Compared with Daqu in Zhoukou area, at the phylum level, the average relative abundance of Actinobacteria and Bacteroidetes in Daqu in Linyi area were significantly lower (P<0.05). At the genus level, the average relative abundance of Acinetobacter was significantly higher (P<0.05), and the average relative abundance of Geobacter and Pantoea were significantly lower (P<0.05). The result of linear discriminant analysis effect size (LEfSe) showed that Geobacter was a biomarker in Zhoukou area Daqu, while the biomarker in Linyi area Daqu was not reflected at the genus level. The result of β diversity showed that there were obvious differences as a whole. The result showed that Daqu in Linyi area was significantly higher than Daqu in Zhoukou area in transcription, amino acid and nucleoside transport and metabolic functions by phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) (P<0.05). Thus, there are obvious differences in bacterial community structure and gene function expression in the two regions.