姜黄联合山楂对C57肥胖小鼠的减肥作用及机制研究

李强; 李亚婷; 徐华健; 郝宗围; 陈鹏浩; 司雄元; 汪雪雁

doi:10.13386/j.issn1002-0306.2023030050

姜黄联合山楂对C57肥胖小鼠的减肥作用及机制研究

Study on the Weight Loss Effect and Mechanism of Turmeric Combined with Hawthorn on C57 Obese Mice

摘要

摘要: 目的：评价姜黄联合山楂的减肥作用并探索其减肥机制。方法：32只C57BL/6J小鼠随机分为四组：正常组、正常干预组、高脂组、高脂干预组，每组8只。每天定时灌胃相应药物，每周定时检测小鼠脂肪率、瘦肉率、摄食和饮水量变化，每隔两周同一时间测定小鼠的随机血糖，12周后脱颈椎处死小鼠采集标本。检测血清总胆固醇（total cholesterol，TC）、甘油三酯（triglyceride，TG）、高密度脂蛋白胆固醇（high-density lipoprotein cholesterol，HDL-C）、低密度脂蛋白胆固醇（low-density lipoprotein cholesterol，LDL-C）、谷丙转氨酶（alanine aminotransferase，ALT）、谷草转氨酶（aspartate aminotransferase，AST），通过附睾脂肪和肝脏HE染色，观察脂肪和肝脏组织形态变化。实时荧光PCR法检测肝脏组织中胆固醇7α-羟化酶（cholesterol 7α-hydroxylase，CYP7A1）、脂肪酸合酶（fatty acid synthase，FAS）、肝X受体α（Liver X Receptorα，LXRα）、固醇调节元件结合蛋白2（Sterol Regulatory Element Binding Protein，SREBP2）、固醇调节元件结合蛋白1C（Sterol Regulatory Element Binding Protein，SREBP1C）、硬脂酰辅酶A去饱和酶（Steary-coenzyme A dehydro-synthase-1，SCD1）mRNA的相对表达水平。结果：相比高脂组，高脂干预组小鼠体质量、脂肪率、TG、TC、LDL-C显著降低，瘦肉率、HDL-C显著升高（P<0.05），摄食、饮水量无显著差异；抑制了脂肪细胞肥大，使得脂肪沉积减少；改善了肝脏细胞空泡化、炎症浸润；肝脏组织中CYP7A1 mRNA表达水平显著升高（P<0.05），LXRα、SREBP1C、SCD、FAS mRNA表达水平极显著降低（P<0.01），SREBP2 mRNA表达水平无显著变化。结论：姜黄-山楂具有良好的减肥作用,其减肥机制与调节相关基因中mRNA表达水平有关。

Abstract: Objective: To evaluate the weight loss effect of turmeric combined with hawthorn and explore its weight loss mechanism. Methods: Thirty-two C57BL/6J mice were randomly divided into four groups: normal group, normal intervention group, high-fat group and high-fat intervention group, 8 mice in each group. The mice were gavaged with the corresponding drugs regularly every day, and the changes of adiposity, leanness, food intake and water consumption were detected regularly every week. The random blood glucose of the mice was measured at the same time every two weeks, after 12 weeks, the mice were removed from the cervical spine and the specimens were collected. The contents of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), aspartate aminotransferase (AST) were measured in serum. The morphological changes of fat and liver tissue were observed by HE staining of epididymal fat and liver. The relative mRNA expressions of cholesterol 7α-hydroxylase (CYP7A1), fatty acid synthase (FAS), Liver X Receptorα (LXRα), Sterol Regulatory Element Binding Protein 2 (SREBP2), Sterol Regulatory Element Binding Protein (SREBP1C), Steary-coenzyme A dehydro-synthase-1 (SCD1) in liver were measured by real time polymerase chain reaction (RT-PCR). Results: Compared with the high-fat group, the body mass, fat percentage, TG, TC, LDL-C of mice in the high-fat intervention group were significantly decreased, lean meat percentage and HDL-C were significantly increased (P<0.05), and there was no significant difference in food intake and water intake. It could inhibit adipocyte hypertrophy and reduce fat deposition. And their liver cell vacuolation and inflammatory infiltration were improved. The mRNA expression level of CYP7A1 in liver was significantly increased (P<0.05). The mRNA expression levels of LXRα, SREBP1C, SCD and FAS were extremely significantly decreased (P<0.01). There was no significant change in the expression level of SREBP2 mRNA. Conclusion: Turmeric-hawthorn has good weight loss effects, and its weight loss mechanism is related to the regulation of mRNA expression levels in related genes.