银柴胡多糖超声辅助提取工艺优化及抗氧化活性分析

王红; 彭励; 宋乐; 冯璐; 李振凯; 李彦青; 高跳

doi:10.13386/j.issn1002-0306.2023030025

银柴胡多糖超声辅助提取工艺优化及抗氧化活性分析

Optimization of Ultrasonic-assisted Extraction Process and Analysis of Antioxidant Activity of Polysaccharide from Stellariae Radix

摘要

摘要: 目的：为了提高银柴胡多糖得率，对银柴胡多糖提取工艺参数进行优化，并评价其体外抗氧化活性。方法：采用超声辅助提取银柴胡多糖，在单因素实验基础上结合响应面法（Box–Behnken Response Surface）对提取工艺参数进行优化，并采用Sevag法除蛋白得银柴胡粗多糖，进一步对其抗氧化活性进行分析。结果：优化后银柴胡多糖最佳提取工艺参数为超声温度50 ℃、时间3.20 h、提取次数2 次，在此条件下多糖得率最高，为28.24%±0.10%，多糖含量为59.13%；体外抗氧化测定结果显示，银柴胡粗多糖清除DPPH自由基、OH自由基、ABTS⁺自由基的IC₅₀分别是5.47、2.40和1.44 mg/mL，表明其具有一定的抗氧化能力。结论：本研究经优化得到的银柴胡多糖提取工艺切实可行，可为银柴胡资源的开发利用提供理论依据。

Abstract: Objective: The aim of this study was to enhance the polysaccharide yield from Stellariae Radix and evaluate its in vitro antioxidant activity. Methods: Ultrasonic-assisted extraction method was employed to extract polysaccharides from Stellariae Radix, the single-factor experiment was combined with the Box-Behnken response surface to optimize the parameters of the extraction process. Additionally, Sevag method was utilized for the removal of protein to obtain crude polysaccharide of Stellariae Radix, which was further analyzed for its antioxidant activity. Results: The optimized conditions for extracting polysaccharides from Stellariae Radix were an ultrasonic temperature of 50 ℃, a time of 3.20 h, and an extraction frequency of 2 times, resulting in a yield of 28.24%±0.10%. The content of polysaccharide was 59.13%. Notably, the crude polysaccharide demonstrated antioxidant activity with IC₅₀ values of 5.47, 2.40 and 1.44 mg/mL for DPPH, OH, and ABTS⁺ radical scavenging activities, respectively. Conclusion: The optimized extraction process of polysaccharides from Stellariae Radix is feasible and can provide a theoretical foundation for the development and utilization of Stellariae Radix.