Abstract: A functional compound selenium-enriched plant solid beverage was successfully developed by taking the selenium-enriched medicinal and edible plants Brassica oleracea, Pueraria lobata, Ampelopsis grossedentata, Polygonatum sibiricum, Chaenomeles sinensis, and tomato as the materials. At the meantime, its biological activities including antiproliferative, antioxidant, and analgesic activities were evaluated. The total selenium content of the compound solid beverage was determined by fluorescence spectrophotometry as 540±30.12 μg/kg. The biological results showed that the compound selenium-enriched plant solid beverage inhibited the proliferation of four cancerous cell lines A549, SMMC-7721, MCF-7, and SW480 with the inhibition values of 4.09%±1.36%, 19.48%±3.18%, 30.75%±1.45%, and 18.20%±1.12%, respectively, and had no cytotoxicity to the human normal lung epithelial cell BEAS-2B at a concentration of 100 μg/mL, indicating its antiproliferative effect against cancer cells in vitro. The compound solid beverage had a clearance ability of DPPH radical at a concentration-dependent manner with an IC₅₀ value of 435.6±27.2 μg/mL. The compound solid beverage reduced the H₂O₂-induced oxidative damage and the intracellular reactive oxygen fluorescence intensity in LO2 cells, and presented a dose-effect relationship within a concentration of 5~20 μg/mL, revealing its obvious antioxidant activity (P<0.01). Additionally, the compound plant selenium solid beverage exhibited significant analgesic activity at the high-dose of 250 mg/kg with an inhibition rate of 92.3% (P<0.001), which was equivalent to that of the positive drug morphine (5 mg/kg). The research results could lay a foundation for further development of the compound selenium-rich plant solid drink.