Abstract: The natural deep eutectic solvent is a new type of green solvent that is expected to replace traditional organic solvents for efficient extraction of natural products such as flavonoids. In order to clarify the application of natural deep eutectic solvents in the extraction of flavonoids, the author summarizes and analyzes relevant research papers published in the past 5 years. This article provides a review of the current research status of natural deep eutectic solvents for extracting flavonoids, and discuss in detail the various factors that affect the extraction rate. The natural deep eutectic solvents perform well in the extraction of various natural flavonoid products such as flavonoids, flavonols, flavonones, anthocyans, and isoflavones. Their extraction rates are generally better than traditional solvents such as methanol and ethanol, and the extracted products have higher activity. The composition, molar ratio, water content, and temperature of deep eutectic solvents significantly affect their extraction of flavonoids. Finally, the development trend of natural eutectic solvents in the future is prospected. This paper aims to provide reference for the efficient and green extraction of flavonoids.