臭氧微纳米气泡处理对黄豆芽“绿化”的影响及机制

高红豆; 史君彦; 刘海涛; 左进华; 袁树枝; 岳晓珍; 王清

doi:10.13386/j.issn1002-0306.2023020059

臭氧微纳米气泡处理对黄豆芽“绿化”的影响及机制

Effect of Ozone Micro-Nano-Bubbles Treatment on “Green” and the Mechanism in Soybean Sprout

摘要

摘要: 为研究臭氧微纳米气泡（ozone micro-nano-bubbles，Ozone MNBs）处理黄豆芽对LED白光照射下黄豆芽“绿化”的影响及其调控机制，本实验以黄豆芽为材料，采用4 mg/L Ozone MNBs处理黄豆芽，测定LED白光照射下黄豆芽生理品质，叶绿素合成和分解相关酶及物质变化。结果表明：与蒸馏水处理对照组相比，4 mg/L Ozone MNBs处理可以显著降低LED白光照射下黄豆芽的“绿化”现象，诱导提高黄豆芽中叶绿素降解酶叶绿素酶（Chlase）、叶绿素降解过氧化物酶（Chl-POX）、脱镁螯合酶（MD）和脱镁叶绿素酶（PPH）活性，降低了叶绿素合成前体物δ-氨基乙酰丙酸（ALA）和尿卟啉原Ⅲ（Urogen Ⅲ），叶绿素、叶绿素a（Chl a）和叶绿素b（Chl b）含量。降低了ADP、ATP和辅酶ⅡNADP⁺和NADPH含量。4 mg/L Ozone MNBs处理可影响黄豆芽叶绿素合成和分解相关酶的活性和相关物质的含量，对抑制LED白光照射下黄豆芽“绿化”有较好的效果。

Abstract: In order to explore the effect of ozone micro-nano-bubbles (Ozone MNBs) on “green” and the regulative mechanism in soybean sprout, this study took soybean sprout as the experimental material, treated with 4 mg/L Ozone MNBs and stored in white LED condition. Physical quality, synthesizing and decomposing of chlorophyll (enzyme activity and substance) were measured in soybean sprout. Compared with control group, 4 mg/L Ozone MNBs treatment could significantly inhibit the “green”, enhance the activities of chlorophyllase (Chlase), chlorophyll degrading peroxidase (Chl-POX), Mg-dechelatase (MD) and pheophytinase (PPH). And it decreased the levels of precursors in chlorophyll synthesis δ-aminolevulinic acid (ALA) and Urogen Ⅲ, chlorophyll, chlorophyll a and chlorophyll b. Additionally, it declined the content of ADP, ATP, NADP⁺ and NADPH in soybean sprout. Thus, 4 mg/L Ozone MNBs treatment affected the substance and enzyme activity of synthesizing and decomposing of chlorophyll, effectively hindered “green” in soybean sprout under white LED.