萸精降糖方对2型糖尿病大鼠血糖血脂及血清氧化应激有关指标的影响

周曦冉; 焦格娜; 曾己; 李博瀚; 沈璐

doi:10.13386/j.issn1002-0306.2022120224

萸精降糖方对2型糖尿病大鼠血糖血脂及血清氧化应激有关指标的影响

Effect of Yujing Jiangtang Recipe on Blood Glucose, Lipid and Serum Oxidative Stress Related Indexes in Type 2 diabetes Rats

摘要

摘要: 目的：研究萸精降糖方对2型糖尿病大鼠血糖血脂及体内抗氧化作用的影响。方法：采用高脂饮食联合腹腔注射40 mg/kg链脲佐菌素（Streptozotocin，STZ）构建2型糖尿病大鼠模型。将造模成功大鼠随机分为糖尿病模型组、二甲双胍组（0.2 g/kg）、萸精降糖方低、中、高剂量组（1、2、4 g/kg），另设正常对照组，每组各9只。治疗组按相应剂量灌胃，正常对照组、糖尿病模型组给予10 mL/kg生理盐水按体重灌胃，各组每天灌胃1次，连续灌胃6周。实验过程中观察并记录各组大鼠体重变化，测定空腹血糖（FBG）；连续灌胃6周后取大鼠腹主动脉血，测定空腹胰岛素（FINS）水平、糖化血红蛋白（HbA1c）百分含量，并计算胰岛素抵抗指数（HOMA-IR）和胰岛素敏感指数（ISI）；测定大鼠血清甘油三酯（TG）、胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）含量；采用酶联免疫吸附测定法（ELISA）测定大鼠血清丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、一氧化氮（NO）、谷胱甘肽过氧化酶（GSH-Px）和过氧化氢酶（CAT）水平；采用苏木精-伊红染色法（HE）对大鼠胰腺组织进行染色，观察大鼠病理形态学的改变。结果：与糖尿病模型组相比，萸精降糖方高剂量组体重极显著升高（P<0.01）；萸精降糖方各剂量组均可极显著降低FBG（P<0.01）；可不同程度降低大鼠FINS、HOMA-IR水平和HbA1c百分含量，升高ISI水平（P<0.05或P<0.01）；可降低血清TG、TC、LDL-C含量和MDA水平（P<0.05或P<0.01），升高HDL-C含量和SOD、GSH水平（P<0.01）；萸精降糖方中、高剂量组可明显升高NO、GSH-Px、CAT水平（P<0.01）；萸精降糖方高剂量组外分泌腺腺泡结构较完整规则，胰岛细胞形态较规则，胰岛内细胞数量较多，排列均匀。结论：萸精降糖方对2型糖尿病大鼠具有降血糖作用，其作用机制可能与调节血脂代谢、改善胰岛素抵抗、抗氧化作用及修复胰腺组织相关。

Abstract: Objective: To study the effect of Yujing Jiangtang Recipe on blood glucose, lipid and antioxidation in type 2 diabetes rats. Methods: High fat diet and intraperitoneal injection of 40 mg/kg STZ were used to establish the model of type 2 diabetes rats. The successful rats were randomly divided into diabetes model group, metformin group (0.2 g/kg), low, medium and high dose Yujing Jiangtang Recipe groups (1、2、4 g/kg), and another normal control group with nine rats in each group. The treatment group was gavaged with corresponding dose, the normal control group and the diabetes model group were gavaged with 10 mL/kg normal saline according to weight, and each group was gavaged once a day for six consecutive weeks. During the experiment, observe and record the weight changes of rats in each group, and measure the fasting blood glucose (FBG). After continuous gavage for six weeks, the abdominal aorta blood of rats was taken, the fasting insulin (FINS) level and the percentage content of glycosylated hemoglobin (HbA1c) were measured, and the HOMA-IR, ISI were calculated. The contents of serum TG, TC, HDL-C, LDL-C were measured. The serum levels of MDA, SOD, GSH, NO, GSH-Px and CAT were measured by ELISA. The pancreatic tissue of rats was stained with HE to observe the pathological changes of rats. Results: Compared with the diabetes model group, the body weight of the high dose group of Yujing Jiangtang Recipe increased significantly (P<0.01). Each dose group of Yujing Jiangtang Recipe could significantly reduce FBG (P<0.01). It could reduce the levels of FINS, HOMA-IR and the percentage of HbA1c in rats to varying degrees, and increase the level of ISI (P<0.05 or P<0.01). It could reduce the content of serum TG, TC, LDL-C and MDA (P<0.05 or P<0.01), and increase the content of HDL-C and the level of SOD and GSH (P<0.01). The levels of NO, GSH-Px and CAT were significantly increased in the middle and high dose groups of Yujing Jiangtang Recipe (P<0.01). In the high-dose group of Yujing Jiangtang Recipe, the structure of exocrine gland acini was relatively complete and regular, the shape of islet cells was relatively regular, and the number of islet cells was large and evenly arranged. Conclusion: Yujing Jiangtang Recipe has hypoglycemic effect on type 2 diabetes rats, and its mechanism may be related to regulating blood lipid metabolism, improving insulin resistance, antioxidation and repairing pancreatic tissue.