基于层次分析-熵权法优化刺五加多组分超声提取工艺

林亚美; 支红欣; 孙霁骧; 陈博宇; 刘胜凯; 刘志国

doi:10.13386/j.issn1002-0306.2022120137

基于层次分析-熵权法优化刺五加多组分超声提取工艺

Optimization of the Ultrasonic Extraction Process of Acanthopanax senticosus Multiple Components Based on a Coupling Methodology of Analytic Hierarchy Process and Entropy Weight Method

摘要

摘要: 基于超高效液相色谱-质谱（ultra-high performance liquid chromatography-mass spectrometry，UPLC-MS/MS）定量方法以及多指标综合评价方法，对刺五加多组分超声提取工艺进行优化。开发UPLC-MS/MS定量方法，同时测定刺五加多组分（绿原酸、紫丁香苷、刺五加苷E、异嗪皮啶、咖啡酸、芝麻素）含量；再由层次分析法（analytic hierarchy process，AHP）及熵权法（entropy weight method，EWM）组建多指标综合评价方法—层次分析-熵权法（AHP-EWM）；最终由单因素结合基于Box-Behnken设计（Box-Behnken design，BBD）的响应面法（response surface methodology，RSM）优化刺五加多组分超声提取工艺。结果表明，RSM优化所得刺五加多组分超声提取最佳工艺：超声功率780 W，超声时间17.5 min，乙醇体积分数57%，料液比1:40 g:mL，原料粒径80目。由此，刺五加根茎中绿原酸、紫丁香苷、刺五加苷E、异嗪皮啶、芝麻素的含量分别为2235.841±12.17、517.959±6.09、861.247±5.30、66.657±1.22、45.745±0.77、99.355±0.69 μg/g，且基于AHP-EWM所得的综合得分为95.45±0.39，与预测理论值接近。刺五加定量分析方法与高效提取工艺的有效开发，为其资源利用以及药效基础研究奠定基础。

Abstract: The multi-component ultrasonic extraction process of Acanthopanax senticosus was optimized via ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS/MS) quantitative method and the multi-index comprehensive evaluation method. An UPLC-MS/MS method for the simultaneous quantitative determination of multiple components (chlorogenic acid, syringin, eleutheroside E, isofraxidin, caffeic acid and sesamin) in rhizome of Acanthopanax senticosus was developed. Then, the analytic hierarchy process (AHP) and entropy weight method (EWM) were combined to establish a multi-index comprehensive evaluation method (AHP-EWM method）. Finally, the multi-component ultrasonic extraction process of Acanthopanax senticosus was optimized by single factor combined with response surface methodology (RSM) based on Box-Behnken design (BBD). The results revealed that the optimal process of ultrasonic extraction of multiple components of Acanthopanax senticosus obtained by RSM optimization was as follows: Ultrasonic power 780 W, ultrasonic time 17.5 min, ethanol volume fraction 57%, solid-liquid ratio 1:40 g:mL, raw material particle size 80 mesh. Therefore, the contents of chlorogenic acid, syringin, eleutheroside E, isofraxidin, caffeic acid and sesamin in rhizome of Acanthopanax senticosus were 2235.841±12.17, 517.959±6.09, 861.247±5.30, 66.657±1.22, 45.745±0.77, 99.355±0.69 μg/g, respectively. The comprehensive score based on the AHP-EWM method was 95.45±0.39, which was close to the predicted theoretical value. The effective development of quantitative analysis method and efficient extraction technology of Acanthopanax senticosus have laid a foundation for its resource utilization and pharmacodynamic basic research.