超声波辅助DES提取肉苁蓉多酚的工艺优化、动力学及抗氧化活性研究

魏玉萍; 赵岩; 宋丽军; 潘磊庆; 侯旭杰

doi:10.13386/j.issn1002-0306.2022100230

超声波辅助DES提取肉苁蓉多酚的工艺优化、动力学及抗氧化活性研究

Optimization of Ultrasonic-Assisted DES Extraction Process, Kinetics and Antioxidant Activity of Cistanche tubulosa Polyphenols

摘要

摘要: 为了研究超声波辅助低共熔溶剂（Deep eutectic solvents，DESs）提取肉苁蓉多酚的最佳工艺条件，以新疆管花肉苁蓉为原料，首先筛选得到脯氨酸-丙三醇（摩尔比2:5）为最佳DES。进一步在单因素实验的基础上采用响应面法优化了超声波辅助DESs提取肉苁蓉多酚的最佳工艺，并对其提取动力学和抗氧化活性进行了研究。结果表明，肉苁蓉多酚的最佳提取工艺为：超声温度40 ℃、液料比45 mL/g、含水率40%、提取时间28 min，在此条件提取率为37.76 mg/g DW。UAE-DES过程符合二级反应动力学。肉苁蓉多酚提取物对DPPH和ABTS⁺自由基清除率的IC₅₀值分别为0.875和0.501 mg/mL。研究结果为新疆管花肉苁蓉多酚的绿色提取和应用提供了理论依据。

Abstract: In order to study the optimal conditions for ultrasonic assisted extraction of polyphenols from Cistanche tubulosa by deep eutectic solvents (DES), the optimal DES (proline:glycerol=2:5) was first selected. Further, response surface methodology (RSM) was used to optimize the extraction processes based on the single factor experiments. The extraction kinetics and antioxidant activity were further studied. The results showed that the optimal conditions were as follows: Ultrasonic temperature of 40 ℃, liquid to solid ratio of 45 mL/g, water content of 40%, extraction time of 28 min. The extraction rate at this condition was 37.76 mg/g DW. Ultrasonic assisted extraction of polyphenols from Cistanche tubulosa conformed to the second-order reaction kinetics. The IC₅₀ values of polyphenol extracts on DPPH and ABTS⁺ free radical scavenging rates were 0.875 and 0.501 mg/mL, respectively. The results would provide a theoretical basis for green extraction and application of polyphenols from Cistanche tubulosa in Xinjiang.