响应面法优化微波辅助提取芦荟蒽醌类物质工艺及其抗氧化活性分析

史友航; 吕青遥; 焦士蓉

doi:10.13386/j.issn1002-0306.2022100192

响应面法优化微波辅助提取芦荟蒽醌类物质工艺及其抗氧化活性分析

Optimization of Microwave-assisted Extraction Process of Anthraquinones from Aloe vera by Response Surface Method and Analysis of Its Antioxidant Activity

摘要

摘要: 为提高芦荟皮中的蒽醌类物质的提取量，本研究采用微波辅助提取法对芦荟蒽醌类物质进行提取，考察不同乙醇浓度、提取时间、微波功率、料液比对提取的影响，以单因素实验为基础，对提取工艺进行响应面优化，并通过其对DPPH自由基清除能力、ABTS⁺自由基清除能力、超氧阴离子自由基清除能力、铁离子还原能力和抑制脂质过氧化物活性的测定，研究芦荟蒽醌类物质的体外抗氧化性。结果表明，微波辅助提取芦荟蒽醌类物质的最优条件为：乙醇浓度为80%、提取时间为60 s、微波功率为406 W、料液比为1:19 g/mL，在此条件下得到的芦荟蒽醌类物质的提取量为12.29 mg/g；其体外抗氧化性的IC₅₀值分别为0.431±0.003、0.400±0.005、0.443±0.012、0.417±0.007、0.441±0.008 mg/mL。

Abstract: In order to improve the extraction amount of anthraquinones from Aloe vera peel, this study used microwave-assisted extraction method to extract anthraquinones from Aloe vera, and investigated the effects of different ethanol concentrations, extraction time, microwave power, and material-to-liquid ratio on the extraction, and carried out response surface optimization of the extraction process based on single-factor experiments, and studied the in vitro antioxidant properties of Aloe vera anthraquinones by its effects on DPPH radical scavenging ability, ABTS⁺ radical scavenging ability, superoxide anion radical scavenging ability, iron ion reducing ability and lipid peroxidation inhibiting activity. The results showed that the optimal conditions for the extraction of Aloe vera anthraquinones by microwave were: Ethanol concentration of 80%, extraction time of 60 s, microwave power of 406 W, and material-to-liquid ratio of 1:19 g/mL. The extracted amount of Aloe vera anthraquinones was 12.29 mg/g, and the in vitro antioxidant IC₅₀ values were 0.431±0.003, 0.400±0.005, 0.443±0.012, 0.417±0.007 and 0.441±0.008 mg/mL, respectively.