Abstract: In this paper, through the direct sleep experiment, the experiment of prolonging the sleep time of pentobarbital sodium, the hypnosis experiment of subthreshold dose of pentobarbital sodium and the sleep latency experiment of pentobarbital sodium, the effects of compound preparations of Ziziphus jujuba extract, longan lour extract, γ-amino butyric acid and casein hydrolysate on sleep improvement in mice were studied. Experiment selected 48 mice as the research object, were randomly divided into control group, high, medium and low dose group, gavage compound preparation after 4 weeks, and the corresponding indicators were measured. The results showed that the compound preparation could significantly prolong the sleep time of mice induced by pentobarbital sodium at high, medium and low doses. The sleep time of the high dose group was 3793±1100 s, the sleep time of the medium dose group was 3591±1589 s, and the sleep time of the low dose group was 3218±582 s, while the sleep time of the control group was only 1556±686 s. The sleep latency of mice hypnotized by barbital sodium was shortened in the high-dose, middle-dose and low-dose compound preparations. The sleep latency was 171±58 s, 165±40 s and 184±52 s respectively. The sleep latency of the control group was 300±155 s. All dose groups of the compound preparation had no significant effect on direct sleep and no significant effect on the body weight of mice.