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中国精品科技期刊2020
黄佳佳,苏妙仪,王如意,等. 红蓝草枝和叶提取物不同萃取部位体外抗氧化和抑菌活性研究[J]. 华体会体育,2023,44(13):102−109. doi: 10.13386/j.issn1002-0306.2022100012.
引用本文: 黄佳佳,苏妙仪,王如意,等. 红蓝草枝和叶提取物不同萃取部位体外抗氧化和抑菌活性研究[J]. 华体会体育,2023,44(13):102−109. doi: 10.13386/j.issn1002-0306.2022100012.
HUANG Jiajia, SU Miaoyi, WANG Ruyi, et al. Antioxidant and Antibacterial Activities of Different Extracted Parts from Branches and Leaves Extracts of the Peristrophe baphica (Spreng.) Bremek[J]. Science and Technology of Food Industry, 2023, 44(13): 102−109. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100012.
Citation: HUANG Jiajia, SU Miaoyi, WANG Ruyi, et al. Antioxidant and Antibacterial Activities of Different Extracted Parts from Branches and Leaves Extracts of the Peristrophe baphica (Spreng.) Bremek[J]. Science and Technology of Food Industry, 2023, 44(13): 102−109. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100012.

红蓝草枝和叶提取物不同萃取部位体外抗氧化和抑菌活性研究

Antioxidant and Antibacterial Activities of Different Extracted Parts from Branches and Leaves Extracts of the Peristrophe baphica (Spreng.) Bremek

  • 摘要: 以红蓝草枝和叶为原料,探究其乙醇提取物和不同溶剂萃取部位的抗氧化及抑菌活性。采用Folin-Ciocalteu法和NaNO2-Al(NO3)3法分别测定乙醇提取物及各萃取部位总酚和黄酮含量;以清除DPPH•、ABTS+•和总还原力为指标评价各部位抗氧化能力并分析抗氧化作用与两种成分之间的相关性。同时,采用牛津杯法测定各部位对常见致病菌的抑菌活性。结果表明,红蓝草枝和叶的乙酸乙酯部位总酚含量最高,分别达79.76 mg/g和80.21 mg/g;枝的氯仿部位和叶的乙醇提取物黄酮含量最高,分别为95.88 mg/g和96.75 mg/g。相关性分析显示红蓝草的抗氧化能力与其酚类含量具有较强相关性。在试验浓度范围内,红蓝草不同组织部位以及不同极性溶剂影响抗氧化及抑菌效果,枝的抗氧化效果优于叶,叶的抑菌效果优于枝。以枝的乙酸乙酯部位,叶的乙酸乙酯和正丁醇部位抗氧化和抑菌效果更为明显,值得进一步研究与开发。

     

    Abstract: In this work, the ethanol extracts of the branches and leaves of Peristrophe baphica (Spreng.) Bremk (PB) as well as the different solvent extracted part of the ethanol extracts were prepared and their antioxidant and antibacterial activities were studied. The contents of total polyphenols and flavonoids of the extracts were determined by Folin-Ciocalteu and NaNO2-Al(NO3)3 method, respectively. The antioxidant activities were evaluated by DPPH radical scavenging ability, ABTS+ radical scavenging ability and total reducing power. The relation between the antioxidant activities and the ethanol extracts as well as the different solvent extracted parts was correlated. The antimicrobial activities of the extracts against several common pathogenic bacteria were determined by Oxford cup method. The results showed that the content of total polyphenols in ethyl acetate part were the highest for both branches and leaves, which was 79.76 mg/g and 80.21 mg/g, respectively. The highest content of flavonoids located in chloroform part of branches and the ethanol extract of leaves, which was 95.88 mg/g and 96.75 mg/g, respectively. The correlation analysis showed that the antioxidant activities of each part were strongly correlated with the contents of total polyphenols. In the test concentration range, different parts of tissue and different solvents had important influence on the antioxidant and antimicrobial activities. The antioxidant activities of branches were better than those of leaves, but the antibacterial activities of leaves were better than those of branches. The ethyl acetate part of branches, the ethyl acetate part and the n-butanol part of leaves showed better antioxidant and antimicrobial activities and could be used for further application.

     

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