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中国精品科技期刊2020
郑新琰,矣润梅,许冬月,等. 不同加工方法对墨红玫瑰花茶产品质量指标及酶活的影响及相关性分析[J]. 华体会体育,2023,44(15):337−345. doi: 10.13386/j.issn1002-0306.2022090328.
引用本文: 郑新琰,矣润梅,许冬月,等. 不同加工方法对墨红玫瑰花茶产品质量指标及酶活的影响及相关性分析[J]. 华体会体育,2023,44(15):337−345. doi: 10.13386/j.issn1002-0306.2022090328.
ZHENG Xinyan, YI Runmei, XU Dongyue, et al. Effect of Different Processing Methods on the Quality Indexes and Enzyme Activities of Rosa Crimson Glory Tea and Correlation Analysis[J]. Science and Technology of Food Industry, 2023, 44(15): 337−345. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090328.
Citation: ZHENG Xinyan, YI Runmei, XU Dongyue, et al. Effect of Different Processing Methods on the Quality Indexes and Enzyme Activities of Rosa Crimson Glory Tea and Correlation Analysis[J]. Science and Technology of Food Industry, 2023, 44(15): 337−345. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090328.

不同加工方法对墨红玫瑰花茶产品质量指标及酶活的影响及相关性分析

Effect of Different Processing Methods on the Quality Indexes and Enzyme Activities of Rosa Crimson Glory Tea and Correlation Analysis

  • 摘要: 目前市面上玫瑰花茶存在加工方法单一,冲泡后香气损失大等问题。本研究参考茶叶加工,将新鲜墨红玫瑰(Rosa crimson glory)花瓣以常温干燥、萎凋发酵干燥、杀青干燥及杀青发酵干燥四种方法加工成花茶,对成品花茶质量指标如感官品质、香气成分、活性物质(总酚、总黄酮、花青素)含量进行对比分析,此外对成品中多酚氧化酶、过氧化物酶酶活进行测定,并将质量指标与两种酶的酶活进行相关性分析。结果表明,杀青发酵干燥法加工出的玫瑰花茶感官品评总分达75.00±8.30,显著高于其他方法加工出的花茶(P<0.05),各因子评分也较高;杀青干燥及杀青发酵干燥法制得的玫瑰花茶主体香气成分如香茅醇含量分别为20838.61±63.06和28218.71±14.95 μg/kg,显著高于萎凋发酵及常温干燥法制得的花茶(P<0.05),此外杀青发酵加工花茶中总酚(184.17±2.04 mg GAE/100 g DW)、黄酮(92.63±2.48 mg芦丁当量/100 g DW)含量较其他加工方式制得的花茶含量显著提高(P<0.05)。但萎凋发酵干燥、杀青干燥、杀青发酵干燥加工均不利于花青素的保留。质量指标与两种酶的酶活间相关性分析结果表明:过氧化物酶酶活与酯类、醛类、醇类、酸类、烷烃类香气成分间均存在负相关关系,而多酚氧化酶与醛类香气成分间存在正相关关系(r=0.602);多酚氧化酶酶活与总酚、总黄酮、花青素等活性物质含量间存在负相关关系,而过氧化物酶则与花青素含量间存在正相关关系(r=0.202),采用杀青加工可有效抑制两种酶的酶活,尤其是多酚氧化酶酶活。总体而言,杀青加工方法制得的玫瑰花茶评分及主体香气成分含量均较高,其中杀青发酵加工方法制得的玫瑰花茶中总酚及总黄酮含量最高;酶活与花茶质量指标间的相关性分析结果可知通过加工方法调控酶活可提升花茶品质。

     

    Abstract: At present, there are some problems in rose tea on the market, such as single processing method, large loss of aroma after brewing, etc. In this study, with reference to tea processing, fresh rose (Rosa crimson glory) petals were processed into scented tea by four methods: Room temperature-drying, withering-fermentation-drying, fixation-drying and fixation-fermentation-drying respectively. The quality indexes of scented tea such as sensory quality, aroma composition, and content of active substances (total phenols, total flavonoids, and anthocyanins) were compared and analyzed. In addition, the enzymatic activities of polyphenol oxidase and peroxidase in the scented tea were determined. Besides, the correlations between the quality indexes and the activities of the two enzymes were analyzed. The results showed that the total sensory evaluation score of rose tea processed by fixation-fermentation-drying treatment was 75.00±8.30, significantly higher than that of rose tea processed by other methods (P<0.05) and it performed better in all factors of sensory quality. The content of the main aroma components, such as citronellol content, in thr rose tea produced by the fixation-drying and fixation-fermentation-drying methods were 20838.61±63.06 and 28218.71±14.95 μg/kg respectively, which was significantly higher than those obtained by withering-fermentation and room-temperature drying methods (P<0.05). In addition, the content of total phenols (184.17±2.04 mg GAE/100 g DW) and flavonoids (92.63±2.48 mg rutin equivalent/100 g DW) in rose tea processed by fixation-fermentation-drying method was significantly increased compared to other processing methods (P<0.05). However, it was unfavorable to the retention of anthocyanins when processed by withering-fermentation-drying, fixation-drying and fixation-fermentation-drying methods. The correlation between the quality indexes and the enzyme activities of the two enzymes showed that there was a negative correlation between the peroxidase enzyme activity and the aroma components of esters, aldehydes, alcohols, acids and alkanes, while there was a positive correlation between the polyphenol oxidase enzyme and the aldehyde aroma components (r=0.602). There was a negative correlation between the polyphenol oxidase enzyme activity and the content of active substances as total phenols, total flavonoids, anthocyanins, while the peroxidase enzyme was negatively correlated with the content of anthocyanins (r=0.202). The enzyme activity of the two enzymes, especially polyphenol oxygenase activity, could be effectively inhibited by fixation processing. In general, the rose tea produced by the method of fixation possessed a higher score and higher main aroma component contents. The rose tea produced by the method of fixation-fermentation-drying method had the highest content of total phenols and flavonoids. The correlation analysis between the enzyme activities and the quality indexes of rose tea showed that the quality of rose tea could be improved by regulating the enzyme activities through processing methods.

     

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