Abstract: Hypertension is a "silent killer" that threatens human health. Milk-derived antihypertensive peptides with antihypertensive activity are prepared from milk protein by enzymatic hydrolysis or fermentation. Recent years, milk-derived antihypertensive peptides have been regarded as a natural and relatively safe bioactive peptide, which have attracted increasing attention due to their high biological activity, low toxicity, easy metabolism and good antihypertensive effect. And milk-derived antihypertensive peptides have been gradually applied to health care products, functional foods and other fields. In this paper, the research progress in the sources (chemically synthesized and natural food sources) and preparation methods (digestibility in vitro and microbial fermentation). This paper summarizes the separation and purification, and structure-activity relationship of milk-derived antihypertensive peptides is reviewed, and the future development prospects for food and clinical applications are outlined, which will provide reference for promoting the development of antihypertensive peptide products in China.