响应面法优化西洋参果多糖的提取工艺及其体外抗氧化活性

赵丽明; 郭煦遥; 毛英民; 赵大庆; 黄宝泰; 李佳奇; 刘莉; 齐滨

doi:10.13386/j.issn1002-0306.2022070318

响应面法优化西洋参果多糖的提取工艺及其体外抗氧化活性

Optimization of Extraction Process and Antioxidant Activity of Polysaccharide from Panax quinquefolium Fruit by Response Surface Methodology

摘要

摘要: 目的：对西洋参果实中的多糖进行提取，结合响应面法对提取工艺进行优化，并对西洋参果多糖是否具有体外抗氧化活性进行研究。方法：本研究以新鲜的西洋参果实为原料，采用了水提醇沉法提取其中的多糖。用单因素实验以及响应面法对提取工艺进行了优化。从DPPH自由基清除率、羟基自由基清除率以及还原能力三个方面进行果多糖的体外抗氧化活性研究。结果：最佳工艺参数为：提取时间为2.5 h，乙醇浓度为80%，料液比为1:16 g/mL，此时的多糖得率为29.47%±0.65%，与模型预测值相当。在以下三方面考察了西洋参果多糖的体外抗氧化活性：多糖浓度为3.4 mg/mL时，其DPPH自由基清除率达75.14%±0.65%，IC₅₀值为0.71 mg/mL；多糖浓度为3.4 mg/mL时，其羟基自由基的清除率可达71.82%±1.43%，IC₅₀值为0.87 mg/mL；多糖的浓度为1.0 mg/mL时，其总还原力达到了0.730，并且其体外抗氧化能力随西洋参果多糖浓度的增加而增强。结论：抗氧化活性的实验结果说明了西洋参果多糖具有较好的抗氧化活性。本研究可以为西洋参果多糖进一步开发利用奠定理论基础，确保中药资源能够被充分利用。

Abstract: Objective: For the extraction of polysaccharides from American ginseng fruits, the extraction process was optimized in combination with response surface methodology, and to investigate whether polysaccharides from American ginseng fruits had antioxidant activity in vitro. Methods: In this study, polysaccharides were extracted from fresh American ginseng fruits by water extraction and alcohol precipitation method. The extraction process was optimized using single factor tests as well as response surface methodology. In vitro antioxidant activity studies of polysaccharides from American ginseng fruits were conducted from three aspects: DPPH radical scavenging rate, hydroxyl radical scavenging rate as well as reducing power. Results: The optimal process parameters were as follows: the extraction time was 2.5 h, the concentration of ethanol was 80%, the feed to liquid ratio of 1:16 g/mL, and the measured average value of polysaccharide yield was 29.47%±0.65%, which was comparable to the model predicted value. In vitro antioxidant activities of polysaccharides from American ginseng fruits were examined in the following three aspects. When the concentration of polysaccharides was 3.4 mg/mL, the DPPH radical scavenging rate was 75.14%±0.65%, and IC₅₀ value was 0.71 mg/mL. When the concentration of polysaccharide was 3.4 mg/mL, the scavenging rate against hydroxyl radicals could reach 71.82%±1.43%, and IC₅₀ value was 0.87 mg/mL. When the concentration of polysaccharides was 1.0 mg/mL, the total reducing power reached 0.730, and its in vitro antioxidant capacity was enhanced with increasing concentrations of polysaccharides from American ginseng fruits. Conclusion: The experimental results of antioxidant activities illustrated that polysaccharides from American ginseng fruits had better antioxidant activities. This study may provide a theoretical basis for further development and utilization of polysaccharides from American ginseng fruits, and ensure that traditional Chinese medicine resources can be fully utilized.