Abstract: In order to obtain high-quality Auricularia auricula glycoprotein and clarify its monosaccharide and amino acid composition, the process conditions of ultrasonic assisted alkaline extraction of Auricularia auricula glycoprotein were optimized by single factor experiment and response surface methodology, and the glycoprotein was separated and purified by AKTAgo purifier and DEAE GE Healthcare chromatographic column, and its monosaccharide and amino acid composition were analyzed by high performance liquid chromatography (HPLC). The optimum extraction conditions of Auricularia auricula glycoprotein were: ultrasonic time 90 min, material liquid ratio 1:90 g/L, ultrasonic power 200 W, pH8.4, extraction temperature 45 ℃, and the comprehensive score of glycoprotein extraction was 55.93. After separation and purification, the purity of the glycoprotein was 74.1% and the protein recovery rate was 37.5%. The monosaccharide composition of the glycoprotein was mainly glucose (9.95 mg/g) and mannose (5.24 mg/g), and the amino acids were mainly L-aspartic acid/L-asparagine (1.16 mg/g), L-phenylalanine (1.14 mg/g) and L-glutamic acid/L-glutamine (1.10 mg/g). Therefore, the ultrasonic alkali method can effectively improve the extraction efficiency of Auricularia auricula glycoprotein, and the Auricularia auricula glycoprotein contains acidic heteropolysaccharides and the necessary amino acids are complete, which provides a scientific reference for the in-depth research and application development of Auricularia auricula glycoprotein.