Abstract: In order to investigate the effect of a high dose of pterostilbene (PTE) on the properties of nanoemulsion, a nanoemulsion delivery system enriched with a high dose of PTE was constructed, and the effect of PTE on the particle size and rheological properties of the emulsion system under different conditions (including pH, salt ion concentration, repeated freeze-thawing, freeze-drying and centrifugation) was investigated. The results showed that a high dose of PTE (2%, w/v) decreased the particle size, increased the absolute value of ζ-potential, the viscosity of the emulsions and the retention rate of the emulsions by centrifugation at 2000~8000 r/min. The high dose of PTE also improved the stability of the nanoemulsion under different pH, low salt ion concentration and freeze-drying treatment conditions. In conclusion, the nanoemulsion not only had a unidirectional effect on the loading of active substance such as PTE, but also the PTE itself affected the nanoemulsion construction and stability. This research wouldprovide a theoretical basis for expanding the application of nanoemulsion loaded with active substances.