四种食用菌菌丝体富集有机钙能力的研究

贺欣宇; 李志鸿; 高谦; 郭欣然; 刘英杰; 王红梅

doi:10.13386/j.issn1002-0306.2022070104

四种食用菌菌丝体富集有机钙能力的研究

Study on the Enrichment Ability of Organic Calcium in Mycelium of Four Edible Fungi

摘要

摘要: 目的：从硫磺菌、茯苓、红曲和蜜环菌中筛选富钙率和钙有机化程度高的食用菌，并研究富钙高的硫磺菌液体培养时富钙对主要活性成分齿孔酸含量的影响。方法：采用生物富集方法研究无机钙的添加对四种食用菌生长的影响，采用静置浸出法分析菌丝体钙有机化程度，并用高效液相色谱法（High Performance Liquid Chromatography, HPLC）分析硫磺菌主要活性成分齿孔酸的含量。结果：固态培养时，在适宜的加钙量下，硫磺菌的生长均优于茯苓、蜜环菌、红曲。液态培养在加钙量为100 mg/L时，硫磺菌的富钙率显著高于茯苓、蜜环菌和红曲。硫磺菌液态培养加钙量为100 mg/L时，可以达到93.51%的富钙率、85.85%的钙有机化程度和18.34 mg/g的齿孔酸含量，因此硫磺菌具有成为功能性新型补钙产品开发利用的潜质。

Abstract: Objective: The edible fungi with high calcium enrichment rate and calcium organic degree were screened from sulfur fungus, Poria cocos, Monascus and Armillaria, and the effect of calcium enrichment on the content of the main active ingredient, dentate acid, was studied when the sulfur fungus with high calcium enrichment were cultured in liquid. Methods: The bioaccumulation method was used to study the effect of inorganic calcium on the growth of four edible fungi. The organic degree of calcium in mycelia was analyzed by static leaching method, and the content of the main active ingredient, dentate acid, was analyzed by HPLC. Results: In solid culture, the growth of sulfur fungus was better than that of Poria cocos, Armillaria and Monascus at the right amount of calcium addition. When the calcium addition amount was 100 mg/L in liquid culture, the calcium enrichment rate of sulfur fungus was significantly higher than those of Poria cocos, Armillaria and Monascus. When the calcium concentration of sulfur fungus liquid culture was 100 mg/L, the calcium enrichment rate was 93.51%, the degree of calcium organization was 85.85%, and the content of the dentate acid was 18.34 mg/g. Therefore, sulfur fungus has the potential to become a new functional calcium supplement product.