Abstract: In order to study the changes of storage quality of red Amygdalus persica treated with melatonin and eugenol, the red Amygdalus persica was used as the experimental material in this experiment. Different treatments (0.5 mmol/L melatonin treatment; 50 µL/L eugenol treatment; 0.5 mmol/L melatonin+50 µL/L eugenol treatment) were used to soak the red Amygdalus persica for 5 min. After treatment, it was naturally dried and stored at a temperature of (0.5±0.5) ℃ to study the quality changes of red Amygdalus persica during storage. The results showed that: Melatonin combined with eugenol treatment could effectively reduce fruit decay rate and respiratory intensity, and delay the decline of firmness, soluble solid content, titratable acid content and total phenol content, effectively maintained SOD activity, APX and PPO activity. At 60 days of storage, the fruit decay rate of melatonin combined with eugenol group was only 14.65%, while that of CK group, melatonin group and eugenol group were 36.72%, 25.63% and 22.49% respectively. In conclusion, melatonin combined with eugenol treatment could delay fruit softening, inhibit the decline of fruit quality and significantly prolong the storage period of fruits.