佛手外皮和果肉的HPLC指纹图谱及多成分定量对比分析

罗华玲; 林海霞; 朱敏凤; 杨丽娟; 王霞

doi:10.13386/j.issn1002-0306.2022050324

佛手外皮和果肉的HPLC指纹图谱及多成分定量对比分析

Comparative Analysis Between Scarfskin and Scarcocarp of Citrus medica L. var. sarcodactylis Swingle by HPLC Fingerprints and Multi-components Content Determination

摘要

摘要: 目的：选取16批次佛手的外皮和果肉作为研究对象，研究二者的差异性。方法：采用HPLC分别建立了佛手外皮和果肉指纹图谱，并进行了多成分的含量测定。结果：果肉中标定了8个共有峰，外皮中标定了18个共有峰，指认了6种成分，分别是东莨菪内酯、滨蒿内酯、橙皮苷、香叶木苷、5,7-二甲氧基香豆素、佛手苷内酯。采用中药色谱指纹图谱相似度评价系统软件进行相似度评价分析，乐山市内佛手外皮和果肉与各自的对照图谱相似度较好，均大于0.90；而同一批次佛手外皮和果肉的相似度非常低只有0.10～0.35。在此基础上，对6种指认的成分进行了含量测定，佛手外皮中，检出6种指认的成分，其中5,7-二甲氧基香豆素、香叶木苷含量较高；而果肉中橙皮苷、香叶木苷含量较高，其余4种成分未检出。结论：通过HPLC指纹图谱及多成分定量分析，发现佛手外皮和果肉的成分及含量存在显著性的差异，利用这些特点，可以将佛手外皮和果肉分别应用于不同的领域，以达到资源利用最大化的目的；同时所建立的方法可用于佛手质量的控制，并为佛手资源的合理高效的开发利用提供了技术支撑。

Abstract: Objective: The scarfskin and scarcocarp of 16 batches of Citrus medica L. var. sarcodactylis Swingle were collected as the research objects to find out the differences between scarfskin and scarcocarp. Methods: The fingerprints of scarfskin and scarcocarp of Citrus medica L. var. sarcodactylis Swingle were established by HPLC, and the content of multicomponents was determined respectively. Result: Eight common peaks were picked up in scarcocarp, 18 in scarfskin, and six constituents were identified as scopoletin, scoparone, hesperidin, diosimin, 5,7-dimethoxy coumarin and bergapten. The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine was employed for similarity analysis. The scarfskin and scarcocarp of Citrus medica L. var. sarcodactylis Swingle in Leshan City had good similarity with their respective reference fingerprints, both greater than 0.90. The similarity between the scarfskin and scarcocarp of the same batch of Citrus medica L. var. sarcodactylis Swingle was very low, only 0.10 to 0.35. On this basis, the contents of six identified components were determined. In the scarfskin of Citrus medica L. var. sarcodactylis Swingle six components were detected, and the contents of 5,7-dimethoxy coumarin and diosimin were higher. But the contents of hesperidin and diosimin in the scarcocarp were higher, and the other four components were not detected. Conclusion: Through HPLC fingerprint and quantitative analysis of multiple components, it was found that there were significant differences in composition and content between scarfskin and scarcocarp. With these characteristics, the scarfskin and scarcocarp of Citrus medica L. var. sarcodactylis Swingle could be used in different fields to maximize the use of resources. At the same time, the established method could be used for quality control of Citrus medica L. var. sarcodactylis Swingle, and would provide technical support for rational and efficient development and utilization of Citrus medica L. var. sarcodactylis Swingle resources.