响应面优化超声波辅助酶法提取湖北海棠叶抗氧化物

吕凯波; 张星雨; 乐薇; 吴士筠; 印彩霞

doi:10.13386/j.issn1002-0306.2022050145

响应面优化超声波辅助酶法提取湖北海棠叶抗氧化物

Optimization of Ultrasound-Assisted Enzymatic Method Extraction of Antioxidant from Malus hupehensis Leaves by Response Surface Methodology

摘要

摘要: 为研究湖北海棠叶中抗氧化物最佳提取工艺，以羟基自由基清除率为参考指标，采用响应面优化超声波辅助酶法对湖北海棠叶抗氧化物提取条件进行研究。结果表明：超声波辅助酶法最佳工艺为超声功率100 W，液料比17:1 mL∙g⁻¹，超声时间32 min，加纤维素酶量2.6%，酶解时间49 min。此条件下羟基自由基清除率为89.9%±0.06%，提取物中黄酮含量14.01%，多酚含量8.93%，多糖含量7.65%，相比煎煮法、回流法、酶法和超声波法，提取物活性物质含量提高了10%以上，DPPH清除率和羟基自由基清除均提高20%以上。利用超声波辅助酶法提取湖北海棠叶抗氧化物效果较好。

Abstract: In order to study the optimal extraction process of antioxidants from Malus hupehensis leaves, hydroxyl radical scavenging rate was taken as a reference index, and the extraction conditions of antioxidants from Malus hupehensis leaves was studied by response surface optimization ultrasonic-assisted enzymatic method. The results showed that the best process of ultrasonic-assisted enzyme method was 100 W ultrasonic power, liquid to material ratio 17:1 mL∙g⁻¹, ultrasonic time 32 min, cellulase addition 2.6%, enzymatic digestion time 49 min. The hydroxyl radical scavenging rate was 89.9%±0.06% under this condition, the flavonoid content in the extract was 14.01%, the polyphenol content was 8.93%, and the polysaccharide content was 7.65%, compared with the decoction method, reflux method, enzymatic method and ultrasonic method, the active substance content was increased by more than 10%, the DPPH scavenging rate and hydroxyl radical scavenging rate were increased by more than 20%. It's better to extraction of antioxidants from Malus hupehensis leaves by ultrasonic aided enzymatic method.