Abstract: Objective: By comparing and analyzing the quality of agate red cherries in different regions of Guizhou Province, the quality differences of these cherries in different areas could be obtained. Methods: The fresh fruits of agate red cherries from Kaiyang, Nayong and Zhijin were picked at the same maturity period, then the fruit weight, soluble solids, total flavonoids, polyphenol and related enzyme activities were measured. Results: From the appearance of the fruit, there was no significant difference in the size of the cherry fruit in the three regions, but the color of the cherry fruit in Nayong was relatively red. In terms of texture, the hardness of cherry fruit in Nayong area was 861.14 g, which was 1.82 times and 1.5 times of Zhijin and Kaiyang respectively. From the nutritional value, the highest free amino acid content of cherry fruit in Zhijin was 46.01 mg∙100 g⁻¹. For the determination of metal ions, the selenium content in Kaiyang area was 153.51 μg/g, which was 11 times of Nayong and 1.27 times of Zhijin. From different aspects, the cherry fruits in the three regions had their own advantages, and the appearance of cherry fruits in Nayong region was relatively good, while the free amino acid and metal ion content of cherry fruits in Zhijin and Kaiyang areas were relatively high. This may be due to the differences in geographical location, climatic conditions and other factors, resulting in certain differences in nutritional value and fruit shape of cherry fruits in the three regions.