Abstract: Glucose detection is particularly important in the food and medical industry. Electrochemical enzyme-free detection and visual detection are widely used in the food industry, biological detection, medical and health and other fields because of its fast, convenient measurement and simple operation. And it has become a hot topic for researchers. This paper summarizes the technology of enzyme-free fast glucose detection at home and abroad. The research progress of enzyme-free glucose sensing technology is reviewed. And the application of these technology is discussed. Among them, the construction simulation enzyme technology in the electrochemical and visual detection are extensive research, discusses their application in clinical medicine and food analysis glucose detection, and the future of glucose enzyme-free detection technology research direction is prospected, for the subsequent development research and application in medical and agricultural industry provides a theoretical basis.