Abstract: The Brassica rapa L. and hawthorn were acted as raw materials to develop the compound beverage of Brassica rapa L. and hawthorn. Subsequently, its effect on exercise endurance of animal was researched. Basing on the result of single factor test, the optimum formula of compound beverage was designed by response surface test. Meanwhile its effect on exercise endurance in Vivo was explored by animal test after for low, medium and high dosage groups were given at 0.1, 0.2 and 0.3 mL/10 g·BW intragastric administration, respectively. The optimal formula of the compound beverage were as follows: Volume proportion of hawthorn juice and polysaccharides solution of Brassica rapa L. was 3:1, dosage of erythritol was 3.4%, dosage of citric acid was 0.23%, dosage of compound stabilizer (sodium carboxymethyl cellulose:xanthan gum:sodium alginate = 2:1:1, mass ratio) was 0.06%. The ultimate beverage presented uniform color, moderate sweet and sour, mixture flavor of Brassica rapa L. and hawthorn, not observed obvious precipitate, the sensory score of beverage was 88.9±0.4. Compared with the results of blank control group, the compound beverage at different dosage could prolonged the exercise time of mice (P<0.05, P<0.01), decreased the LA accumulation and decomposition of protein or amino acid (P<0.05, P<0.01), and enhanced activity of SOD and GSH-Px (P<0.05, P<0.01). Therefore, it could contribute to improve exercise endurance of body.