Abstract: In order to study the effect of different film packaging on storage quality and physiological characteristics of pitaya fruit, with 'Zihonglong' pitaya as the experimental material, three film packaging bags of polyethylene fresh-keeping bag (PE), polyvinyl chloride fresh-keeping bag (PVC), and polyethylene microporous fresh-keeping bag (WK) were used to process and package the fruit. Then they were stored at (6±1) °C. The results illustrated that compared with the control (CK), the different film packaging could inhibit the increase of pitaya fruit decay rate and weight loss rate, and delay the appearance of respiration peak of pitaya fruit, inhibit the decrease of the total soluble solids (TSS), titratable acid (TA), soluble protein and soluble sugar, vitamin C (V_C) and glutathione (GSH), reduce the accumulation of malondialdehyde (MDA), increase superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities. The decay rates of pitaya fruit in groups CK, PVC, WK, PE were 44.44%, 33.33%, 22.22% and 11.11%, respectively after storage for 30 d. PE film packaging had the best preservation effect, which could effectively delay the deterioration of pitaya after harvest and was more conducive to the maintenance of fruit quality.