Abstract: The process of cold chain of fruits and vegetables can easily cause damage to the internal structure of fruits and vegetables and arouse quality deterioration due to problems such as extensive control and large losses. Meanwhile, the moisture and heat inside the fruits and vegetables would dynamically migrate, which is not a beneficial effect of the storage and sales on fruits and vegetables. Therefore, it is an urgent problem for studying multi-scale heat and mass transfer mechanism in the cold chain process of fruits and vegetables. This paper clarifies the numerical imaging technology for multi-scale modeling of fruits and vegetables, discusses the multi-scale computational fluid dynamics modeling method of heat and mass transfer mechanism in the cold chain process of fruits and vegetables, as well as the basic problems and solutions in modeling, and optimizes the heat and mass transfer models and designs more efficient and reasonable cooling systems, in order to provide a theoretical basis for the study of the multi-scale heat and mass transfer mechanism, loss reduction and preservation during the cold chain process of fruits and vegetables.