茶多糖-茶多酚对D-半乳糖诱导的小鼠脑组织氧化损伤的改善作用

童鑫怡; 韦铮; 陈梅; 程鸿; 黄先智; 丁晓雯

doi:10.13386/j.issn1002-0306.2021110008

茶多糖-茶多酚对D-半乳糖诱导的小鼠脑组织氧化损伤的改善作用

Effect of Tea Polysaccharide-Tea Polyphenol on Improving D-Galactose-Induced Oxidative Damage of Brain in Mice

摘要

摘要: 目的：探究茶多糖-茶多酚混合成分（茶多糖含量为56.93%，茶多酚含量为19.37%）对D-半乳糖（D-galactose，D-Gal）诱导的小鼠脑组织氧化损伤的改善作用，为功能性食品的研发提供理论依据。方法：以D-Gal诱导小鼠建立氧化应激损伤模型，保留12只小鼠作为正常组。将建模成功的小鼠随机分为模型对照组、阳性药物组（还原型谷胱甘肽，200 mg/kg·bw）、茶多酚对照组（50 mg/kg·bw，与茶多糖-茶多酚高剂量组中茶多酚剂量相当）以及茶多糖-茶多酚混合成分的低、中、高剂量组（40、100、250 mg/kg·bw），连续灌胃45 d，测定小鼠脑组织匀浆中生物大分子氧化损伤标志物含量及酶类抗氧化系统相关指标的变化。结果：与模型组对比，茶多糖-茶多酚高剂量组小鼠脑组织匀浆中蛋白质羰基（protein carbonyl，PCO）、晚期蛋白氧化产物（advanced oxidation，AOPP）、3-硝基酪蛋白（3-nitrotyrosine，3-NT）、丙二醛（malondialdehyde，MDA）、8-异前列腺素（8-iso-prostaglandin，8-iso-PG）、8-羟基脱氧鸟嘌呤（8-hydroxy-2'-desoxyguanosine，8-OHdG）、5-羟基胞嘧啶（5- hydroxy-2'- desoxyguanosine，5-OH-DG）分别降低22.95%、15.23%、15.29%、25.23%、23.15%、32.36%、28.63%（P<0.05）；超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GPX）水平分别增加71.15%（P<0.05）、36.90%（P<0.05），均恢复到正常水平（P>0.05）。与茶多酚组相比，茶多糖-茶多酚高剂量组的PCO、AOPP、3-NT、MDA、8-iso-PG、5-OH-DG水平分别降低4.06%、1.81%、4.96%、10.12%、3.40%、12.50%（P>0.05），8-OHdG水平下降21.19%（P<0.05）；SOD浓度增加15.63%（P>0.05），GPX浓度增加5.84%（P<0.05）。结论：茶多糖-茶多酚混合成分能有效改善小鼠脑组织的氧化损伤，且茶多糖-茶多酚混合成分的改善作用优于同剂量茶多酚。

Abstract: Objective: To explore the synergistic effect of tea polysaccharide-tea polyphenol on the improvement of oxidative stress damage in brain induced by D-galactose (D-Gal) in mice, and to provide a basic for the development of functional food. Methods: The oxidative stress model was induced by D-Gal, and 12 mice were retained as the normal control group. The mice that were successfully modeled were randomly divided into a model control group and a positive drug group (reduced glutathione, 200 mg/kg·bw), tea polyphenol group (50 mg/kg·bw), and low-dose, medium-dose and high-dose tea polysaccharide-tea polyphenol groups (40, 100, 250 mg/kg·bw). Gavage for 45 days to determine the content of biomacromolecule oxidative damage markers and enzyme antioxidant system related indicators in the brain of homogenate mice. Results: Compared with the model control group, the levels of the high-dose tea polysaccharide-tea polyphenol group of the biomacromolecule oxidative damage markers including protein carbonyl (PCO), advanced oxidation (AOPP), 3-nitrotyrosine (3-NT), malondialdehyde (MDA), 8-iso-prostaglandin(8-iso-PG), 8-hydroxy-2'-desoxyguanosine (8-OHdG), 5- hydroxy-2' -desoxyguanosine (5-OH-DG) were significantly decreased by 22.95%, 15.23%, 15.29%, 25.23%, 23.15%, 32.36%, 28.63%, respectively (P<0.05). The levels of antioxidants including SOD and GPX were significantly increased by 71.15% (P<0.05) and 36.90% (P<0.05), all of which reached the normal levels (P>0.05). Compared with the tea polyphenol group, the levels of PCO, AOPP, 3-NT, MDA, 8-iso-PG, 8-OHdG, 5-OH-DG of the high-dose tea polysaccharide-tea polyphenol group were decreased by 4.06%, 1.81%, 4.96%, 10.12%, 3.40%, 12.50%(P>0.05), respectively and the level of 8-OHdG was significantly decreased by 21.19% (P<0.05). The level of SOD was increased by 15.63% (P>0.05) and the level of GPX was significantly increased by 5.84% (P<0.05). Conclusion: The tea polysaccharide-tea polyphenol mixture could effectively improve the oxidative damage of the brain of mice, and the effect of the tea polysaccharide-tea polyphenol mixture was better than that of the same dose of tea polyphenol.