Abstract: Using green and highly efficient deep eutectic solvent (DES) as the extraction agent, this study extracted the functional ingredient—cyclic adenosine 3',5'-monophosphate (cAMP) from Xinjiang jujube through ultrasound-assisted technology. The relationships between the molar ratio of deep eutectic solvent, water content of deep eutectic solvent, solid-liquid ratio, ultrasonic time, ultrasonic temperature and extraction amount of cAMP were studied. Through single factor experimentas and response surface optimization experiment, the optimal conditions for cAMP extraction from Xinjiang jujube were as follows: The molar ratio of choline chloride to glycerol was 1:3, the water content of DES system was 44%, the solid-liquid ratio of jujube powder to DES was 1:35 g/mL, the ultrasonic time was 45 min, and the ultrasonic temperature was 45 ℃. At this time, compared with water extraction and alcohol extraction under the same ultrasonic conditions, the highest content of cAMP extracted by deep eutectic solvent was (284.15±0.06) μg/g. Therefore, ultrasonic-assisted deep eutectic solvent extraction of cAMP in Xinjiang jujube was a new, efficient and safe method to obtain a higher extraction amount.