酸枣提取物的抗氧化及抗衰老作用

叶晓楠; 曾慧慧; 刘思琦; 李毅翔; 黄莉莉; 于爽; 李廷利; 王艳艳

doi:10.13386/j.issn1002-0306.2021090192

酸枣提取物的抗氧化及抗衰老作用

Antioxidant and Anti-aging Activity of Ziziphus jujuba Extract

摘要

摘要: 目的：研究酸枣提取物体外和体内抗氧化及抗衰老作用。方法：对不同质量浓度酸枣提取物（0.05、0.10、0.20、0.40、0.60、0.80、1.00 mg/mL）进行1,1-二苯基-2-三硝基苯肼（DPPH）自由基清除率、羟自由基清除率及Fe³⁺还原能力测定，评价酸枣提取物体外抗氧化活性；以不同质量分数（0.1%、0.5%、1.0%）酸枣提取物饲喂果蝇，通过果蝇寿命实验、逆重力爬行实验以及测定果蝇体内超氧化物歧化酶（Superoxide dismutase，SOD）活力、丙二醛（Malonic dialdehyde，MDA）含量、过氧化氢酶（Catalase，CAT）活力，评价酸枣提取物体内抗氧化及抗衰老作用。结果：体外实验结果显示，酸枣提取物对DPPH自由基、羟自由基均有不同程度的清除作用，其半数清除浓度（IC₅₀）值分别为：0.44、1.40 mg/mL，对Fe³⁺具有还原能力。体内实验结果显示，与空白组相比，1.0%酸枣提取物组雌、雄果蝇平均寿命分别显著延长了9.61%和13.91%（P<0.01）；增强30 d雌、雄果蝇逆重力爬行能力（P<0.05或P<0.01），降低雌、雄果蝇体内MDA含量（P<0.05或P<0.01），提高雌、雄果蝇体内SOD和CAT活力（P<0.05或P<0.01）。结论：酸枣提取物具有较好的体内外抗氧化作用和潜在抗衰老作用。

Abstract: Objective:To illustrate the antioxidant and anti-aging activity of Ziziphus jujuba extract in vivo and in vitro. Methods: The antioxidant activity of Ziziphus jujuba extract (0.05、0.10、0.20、0.40、0.60、0.80、1.00 mg/mL) was determined by DPPH free radical scavenging rate, ·OH radical clearance rate and Fe³⁺ reducing antioxidant power assayed in vitro. Three different doses of Ziziphus jujuba extract (0.1%、0.5%、1.0%) were given to the Drosophila melanogaster through the culture to study the in vivo antioxidant and anti-aging activity by testing the lifespans, negative geotaxis ability, SOD activity, MAD content and CAT activity. Results: The results of in vitro antioxidant activity showed that the Ziziphus jujuba extract could scavenge DPPH and OH free radicals in vitro, and its IC₅₀ were 0.44 and 1.40 mg/mL, respectively. It also could reduce iron in vitro. The in vivo antioxidant and anti-aging activity results showed that the average life span of female and male D. melanogasterwere prolonged by 9.61% and 13.91% in 1.0% Ziziphus jujuba extract group compared with the control group (P<0.01). Also Ziziphus jujuba extract treatment improved the negative geotaxis ability in older female and male D. melanogaster (P<0.05 or P<0.01), decreased the MAD content (P<0.05 or P<0.01) and increased the SOD and CAT activity (P<0.05 or P<0.01). Conclusion: The Ziziphus jujuba extract has good antioxidant and anti-aging capacity in vivo and in vitro.