黄蜀葵花总黄酮提取工艺优化及其对黄嘌呤氧化酶的抑制作用

王敏; 徐国辉; 赵一灵; 王刚强; 朱湘玥; 黄赣辉

doi:10.13386/j.issn1002-0306.2021090191

黄蜀葵花总黄酮提取工艺优化及其对黄嘌呤氧化酶的抑制作用

Optimization of Extraction Process for Total Flavonoids from Abelmoschus manihot and Its Inhibitory Effect on Xanthine Oxidase

摘要

摘要: 通过十二烷基硫酸钠（SDS）协同超声波提取黄蜀葵花中总黄酮，并研究其对黄嘌呤氧化酶（XOD）的抑制作用，从而达到高效利用黄蜀葵花资源的目的。以总黄酮含量为指标，通过单因素实验确定料液比、超声波功率、SDS浓度为主要影响因素，再采用Box-Behnken中心组合设计结合响应面分析法，对黄蜀葵花总黄酮提取工艺进行优化，并对提取液抑制XOD的效果进行测定。结果表明：最佳提取方案为料液比1:48 g/mL，超声功率320 W，SDS浓度0.05 g/mL，在此条件下对黄蜀葵花中总黄酮进行提取，含量为4.63 mg/g；在抑制剂浓度为0.38~1.92 mg/mL范围内，黄蜀葵花提取液XOD抑制率最高为98.35%，对XOD有良好的抑制作用。

Abstract: Total flavonoids from Abelmoschus corolla were extracted by sodium dodecyl sulfate (SDS)-assisted ultrasonic wave, and its inhibition of xanthine oxidase (XOD) was studied, so as to achieve the efficient utilization of the resources of Abelmoschus manihot. Taking the content of total flavonoids as the index, the solid/liquid ratio, ultrasonic power and concentration of SDS were determined by single factor experiment. The extraction process of total flavonoids was optimized by combining Box-Behnken center and response surface analysis, and the effect of the extract on inhibiting XOD was determined. The results showed that the optimal extraction method was as follows: The solid/liquid ratio was 1:48 g/mL, the ultrasonic power was 320 W, the concentration of SDS was 0.05 g/mL. Under these conditions, the total flavonoids in Abelmoschus corolla were 4.63 mg/g. In the range of inhibitor concentration of 0.38~1.92 mg/mL, the inhibition rate of extracting solution was 98.35%, which had a good inhibitory effect on XOD.