Abstract: Natural starch mainly consists of two forms of α-D-glucan, i.e. amylose and amylopectin. The ratio of these two components is not only related to gelatinization, retrogradation, gelation and other physicochemical properties and food functions of starch, but also determines the economic value of starch. This means, the quantification technologies of amylose/amylopectin ratio determination can be widely used in grain crop breeding, high-value utilization of starch and the quality assurance and innovation of food. In this paper, the diverse demands of the quantification of amylose/amylopectin ratio in the field of food science research and production are reviewed. The principles, pros and cons, and applications of various classic and novel quantitative technology of amylose/amylopectin ratio are reviewed and compared. The innovative application of the quantitative technology of amylose/amylopectin ratio in future food-related fields is also prospected.