山西毛建茶水提物解酒保肝作用研究

李会芳; 郎霞; 程生辉; 王智深

doi:10.13386/j.issn1002-0306.2021080205

山西毛建茶水提物解酒保肝作用研究

Anti-alcoholic and Hepatoprotective Effect of Shanxi Maojian Tea Aqueous Extract

摘要

摘要: 目的：研究山西毛建茶水提物的解酒保肝作用，为其进一步开发利用提供依据。方法：将昆明小鼠随机分为 5 组：醉酒模型组、盐酸纳洛酮组（0.002 g/kg）、毛建茶高剂量组（3 g/kg）、毛建茶中剂量组（1.5 g/kg）和毛建茶低剂量组（0.75 g/kg）。毛建茶各组单次灌胃后2 h给予50%酒精（20 mL/kg），盐酸纳洛酮组先给予50%酒精30 min后腹腔注射给予盐酸纳洛酮注射液，记录各组醉酒小鼠的醉酒时间和醒酒时间。将SD大鼠60只，随机分为6组：空白组、慢性酒精性肝损伤模型组、东宝肝泰组（0.36 g/kg）、毛建茶高、中、低剂量组，除空白组外各组上午灌服50%酒精；下午东宝肝泰组和毛建茶各剂量组灌胃给予相应药物，连续给药6周。实验结束后，留存血清检测谷氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、胆固醇（TC）、甘油三脂（TG）水平，肝脏中丙二醛（MDA）、超氧化物歧酶（SOD）、谷胱甘肽（GSH）水平，对大鼠肝脏H&E染色进行病理学观察。结果表明：与醉酒模型组相比，毛建茶各剂量组醒酒时间极显著缩短（P<0.01）。与空白组相比，慢性酒精性肝损伤模型组大鼠ALT、AST、TC、TG水平显著升高（P<0.05），MDA水平极显著升高（P<0.01），SOD、GSH水平极显著降低（P<0.01）；与慢性酒精性肝损伤模型组相比，毛建茶高剂量组AST、TC、MDA水平极显著降低（P<0.01），SOD、GSH水平极显著升高（P<0.01），毛建茶中剂量组ALT水平显著降低（P<0.05）、AST水平极显著降低（P<0.01），SOD、GSH水平极显著升高（P<0.01），毛建茶低剂量组AST、TG水平极显著降低（P<0.01），SOD、GSH水平极显著升高（P<0.01），H&E染色结果显示毛建茶水提物各剂量组可不同程度改善肝组织的病理变化。结论：毛建茶水提物具有一定的解酒保肝作用。

Abstract: Objective: To study the anti-alcoholic and hepatoprotective effect of Shanxi Maojian tea aqueous extract, in order to provide the evidence for its further development and utilization. Methods: Kunming mice were randomly divided into 5 groups: Drunken model group, naloxone hydrochloride group (0.002 g/kg), high-dose Maojian tea group (3 g/kg), medium-dose Maojian tea group (1.5 g/kg), low-dose Maojian tea group (0.75 g/kg). Two hours after single gavage of Maojian tea by dosage of administration, each group was given the corresponding 50% alcohol (20 mL/kg), and 30 minutes after gavage with 50% alcohol, naloxone hydrochloride group was intraperitoneally injected with naloxone hydrochloride injection. The ebriety time and the sober time of drunken mice in each group were recorded. Sixty SD rats were randomly divided into 6 groups: Control group, chronic alcoholic liver injury model group, Dongbaogantai group (0.36 g/kg), Maojian tea high-dose, medium-dose and low-dose groups. Except for control group, the other groups gavage with 50% alcohol in the morning. In the afternoon, Dongbaogantai group and Maojian tea groups were given the corresponding drugs by continuous administration for 6 weeks. After the experiment, the contents of glutamate aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol (TC), triglyceride (TG) in serum were detected, and the levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) in liver were detected and pathological observation was taken after H&E staining with liver of rats. Results: Compared with the drunken model group, the sober time of Maojian tea groups were significantly shortened (P<0.01). Compared with control group, the levels of ALT, AST, TC, TG in chronic alcoholic liver injury model group were significantly increased (P<0.05), and MDA significantly increased (P<0.01), while the levels of SOD and GSH were significantly decreased (P<0.01). Compared with alcoholic liver injury group, the levels of AST, TC and MDA in Maojian tea high-dose group were significantly decreased (P<0.01), the levels of SOD and GSH were significantly increased (P<0.01), and the levels of ALT in Maojian tea medium-dose group were significantly decreased (P<0.05), AST content significantly decreased (P<0.01), SOD and GSH significantly increased (P<0.01), the levels of AST and TG in Maojian tea low-dose group were significantly decreased (P<0.01), and the levels of SOD and GSH were significantly increased (P<0.01). H&E staining results showed that the pathological changes of liver tissues in different doses of Maojian tea groups could be improved to different degrees. Conclusion: Maojian tea had anti-alcoholic and hepatoprotective effect.