大孔吸附树脂分离纯化海蜇ACE抑制肽的工艺研究

崔婷婷; 贾爱荣; 张绵松; 刘雪; 白义化; 苗佳琳; 刘昌衡

doi:10.13386/j.issn1002-0306.2021080193

大孔吸附树脂分离纯化海蜇ACE抑制肽的工艺研究

Separation and Purification of ACE Inhibitory Peptide from Jellyfish by Macroporous Resin

摘要

摘要: 本文主要从分子极性角度研究了大孔吸附树脂对具有血管紧张素转化酶（Angiotensin-I converting enzyme，ACE）抑制活性的海蜇多肽的分离纯化作用。取海蜇酶解产物作为研究对象，选用HP20SS、SP20SS、SP207三种不同型号的大孔吸附树脂分离纯化海蜇ACE抑制肽，以ACE抑制率为评价指标，对其分离纯化海蜇ACE抑制肽的工艺进行研究。结果发现，HP20SS型大孔吸附树脂分离纯化海蜇ACE抑制肽效果最佳，当海蜇酶解液的浓度为10.0 mg/mL、上样流速为2.0 BV/h、静置吸附3 h、洗脱剂为70%的乙醇溶液、洗脱剂流速为1.0 mL/min的条件下，富集后的ACE抑制肽分子量为2.65×10³ Da，纯度为89.16%，抑制率高达92.18%，IC₅₀值为1.02 mg/mL。经3次平行试验，样品的平均回收率为94.76%，RSD为0.63%。综合分析，HP20SS大孔吸附树脂对海蜇ACE抑制肽有较好的分离纯化效果，该工艺合理可行且重现性好。

Abstract: In this paper, the separation and purification of jellyfish polypeptide with Angiotensin-I converting enzyme (ACE) inhibitory activity by macroporous adsorption resin was studied from the perspective of molecular polarity. The enzymatic hydrolysates of jellyfish were taken as the research object, and three different types of macroporous resin HP20SS, SP20SS and SP207 were used to isolate and purify the ACE inhibitory peptide of jellyfish. The ACE inhibitory rate was used as the evaluation index, and the separation and purification process of ACE inhibitory peptide was screened. The results showed that HP20SS macroporous resin was the best for the separation and purification of ACE inhibitory peptide. Under the conditions of 10.0 mg/mL of hydrolysate, 2.0 BV/h of sample loading, 3 h of standing adsorption, 70% ethanol solution of eluent, 1.0 mL/min of eluent, after enrichment, the molecular weight of ACE inhibitory peptide was 2.65×10³ Da, the purity was 89.16%, and the inhibition rate was up to 92.18%, the IC₅₀ value was 1.02 mg/mL. The average recovery was 94.76% and RSD was 0.63% after three parallel tests. Comprehensive analysis showed that HP20SS macroporous resin had a good separation and purification effect on the ACE inhibitory peptide of jellyfish, and the process was reasonable and feasible with good reproducibility.