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中国精品科技期刊2020
赵齐燕,唐宁,贾鑫,等. 两类紫云英苗菜生长过程中酚类物质及其抗氧化活性的变化[J]. 华体会体育,2022,43(18):12−20. doi: 10.13386/j.issn1002-0306.2021070340.
引用本文: 赵齐燕,唐宁,贾鑫,等. 两类紫云英苗菜生长过程中酚类物质及其抗氧化活性的变化[J]. 华体会体育,2022,43(18):12−20. doi: 10.13386/j.issn1002-0306.2021070340.
ZHAO Qiyan, TANG Ning, JIA Xin, et al. Changes in Phenolics and Antioxidant Capability of Two Chinese Milk Vetch Sprouts During Germination[J]. Science and Technology of Food Industry, 2022, 43(18): 12−20. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070340.
Citation: ZHAO Qiyan, TANG Ning, JIA Xin, et al. Changes in Phenolics and Antioxidant Capability of Two Chinese Milk Vetch Sprouts During Germination[J]. Science and Technology of Food Industry, 2022, 43(18): 12−20. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070340.

两类紫云英苗菜生长过程中酚类物质及其抗氧化活性的变化

Changes in Phenolics and Antioxidant Capability of Two Chinese Milk Vetch Sprouts During Germination

  • 摘要: 紫云英营养价值高,常做绿肥、牧草使用,种植效益较低。为拓宽紫云英资源化利用渠道,选取湘紫1号、皖紫1号两类紫云英种子水培开发苗菜,以土培紫云英成熟茎叶为对照,对比分析两种种质酚类物质组成差异和发芽8~14 d的变化,以及酚类物质与抗氧化性之间的相关性。结果发现,两类紫云英苗菜生长过程中总酚、总黄酮含量以及抗氧化能力均呈上升趋势;皖紫1号苗菜酚类物质含量以及抗氧化能力均优于湘紫1号。发芽14 d的皖紫1号苗菜总酚(7.31 mg/g dw)、总黄酮(5.86 mg/g dw)含量高且抗氧化性强,共检测出12种酚类化合物,比土培成熟茎叶多检测出没食子酸、阿魏酸、香草酸、水杨酸、丁香酸5种酚类物质。其优势成分为山奈酚(12.86±0.34 mg/g)、芹菜素(6.38±0.17 mg/g)、槲皮素(5.36±0.04 mg/g)、对香豆酸(4.59±0.13 mg/g)、没食子酸(3.01±0.11 mg/g)、木犀草素(3.00±0.02 mg/g)和阿魏酸(2.35±0.29 mg/g)。其中,没食子酸含量为发芽14 d湘紫1号苗菜13倍,对香豆酸含量达到土培成熟茎叶的33倍。本文为紫云英苗菜选种、育苗提供了初步理论支撑和相关参数参考,建议增强对紫云英水培开发苗菜和其酚类资源的关注和开发。

     

    Abstract: Chinese milk vetch (CMV) has high nutritional value, but it is often used as green manure and forage grasses, with low economic returns. This study aimed to broaden the channel of its resource utilization. Two CMV seeds from Hunan (Xiang No.1) and Anhui (Wan No.1) province were hydroponically developed to CMV sprouts. Taking mature stems and leaves of CMV as the positive control, the differences in phenolic composition and dynamic changes of antioxidant capability during 8~14 d after germination were analyzed and compared between two sprouts, as well as the correlation between phenolic substances and antioxidant activity. The results showed that the contents of total phenols (TPC), total flavonoids (TFC) and antioxidant capacity of two CMV sprouts had an upward trend during 8~14 d of growth. Wan No.1 sprouts performed better than Xiang No.1 sprouts. The 14th-day Wan No.1 sprouts showed high TPC (7.31 mg/g dw), TFC (5.86 mg/g dw) and strong antioxidant activities. A total of 12 phenolic compounds were detected, including five more phenolic substances than mature stems and leaves, which were gallic acid, ferulic acid, vanillic acid, salicylic acid and syringic acid. Its dominant components were kaempferol (12.86±0.34 mg/g), apigenin (6.38±0.17 mg/g), quercetin (5.36±0.04 mg/g), p-coumaric acid (4.59±0.13 mg/g), gallic acid (3.01±0.11 mg/g), luteolin (3.00±0.02 mg/g) and ferulic acid (2.35±0.29 mg/g). Among them, the content of gallic acid was 13 times that of the 14th-day Xiang No.1 sprouts, and the content of p-coumaric acid was 33 times that of mature stems and leaves. This study could provide preliminary theoretical support and related parameters for CMV seed selection and sprouts breeding, and it is recommended that CMV sprouts should be further focused and studied.

     

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