胶原蛋白肽与银耳多糖对衰老小鼠皮肤改善作用及机理研究

徐晓飞; 钟兴伟; 赵明月; 何瑞琪

doi:10.13386/j.issn1002-0306.2021060067

胶原蛋白肽与银耳多糖对衰老小鼠皮肤改善作用及机理研究

Research on the Improvement Effects of Collagen Peptides and Tremella fuciformis Polysaccharides on the Skin Texture of Aging Mice and Its Mechanism

摘要

摘要: 目的：比较胶原蛋白肽和银耳多糖对衰老皮肤改善效果及探究作用机理。方法：以120 mg/kg D-半乳糖腹腔注射构建衰老小鼠模型，分为模型组、胶原蛋白肽组、银耳多糖组，同时分别灌胃生理盐水、胶原蛋白肽830 mg/kg·BW和银耳多糖100 mg/kg·BW，连续8周，正常小鼠作为对照组。实验结束后取小鼠背部皮肤检测羟脯氨酸和透明质酸含量，取组织测定超氧化物歧化酶（Superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（Glutathione peroxidase，GSH-Px）活性及丙二醛（Malondialdehyde，MDA）浓度，以及检测炎性细胞因子水平及肠道中短链脂肪酸（short chain fatty acids，SCFAs）浓度。结果：在试验剂量下，胶原蛋白肽和银耳多糖都能极显著提升衰老皮肤中羟脯氨酸和透明质酸含量（P<0.01），且银耳多糖优于胶原蛋白肽。进一步检测发现胶原蛋白肽和银耳多糖极显著提高了血清、肝脏和心脏部位GSH-Px、SOD活性并降低MDA浓度（P<0.01），两者作用效果无差异，但银耳多糖降低血清中IL-1β、TNF-α的效果比胶原蛋白肽更好（P<0.01）。对肠道中6种SCFAs检测表明，胶原蛋白肽对衰老小鼠结肠部位总SCFAs无明显影响，但增加了乙酸、丙酸浓度，而银耳多糖则逆转了D-半乳糖所致结肠部位SCFAs浓度的改变。结论：胶原蛋白肽和银耳多糖对小鼠衰老皮肤的改善作用可能是通过提高机体抗氧化酶活性和抗炎作用而实现。

Abstract: Objective: To compare the improvement effects of collagen peptides and Tremella fuciformis polysaccharides (TFP) on aging skin texture and explore the underlying mechanism of the action. Method: Aging mice models were established by intraperitoneal injection of D-galactose at a dosage of 120 mg/kg, which were randomly divided into three groups: Model (given saline), collagen (given collagen peptides 830 mg/kg·BW), and TFP (given TFP 100 mg/kg·BW). Adult mice were set as normal group (Normal). The experiments lasted for 8 weeks. All mice were killed at the end of the experiment. The contents of hydroxyproline and hyaluronic acid in the skin, the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and concentrations of malondialdehyde (MDA) in several tissues, the levels of inflammatory cytokines in serum, and the concentrations of intestinal short chain fatty acids (SCFAs) were measured. Results: Collagen peptides and TFP significantly increased the contents of hydroxyproline and hyaluronic acid in aging skin in mice (P<0.01), and the improving effects of TFP were better than that of collagen peptides. Further, both of collagen peptides and TFP significantly improved the activities of GSH-Px and SOD, and reduced the concentrations of MDA (P<0.01) in serum, liver, and heart, but there was no difference between Collagen group and TFP group. Interestingly, TFP showed a better inhibitory effect on the concentrations of IL-1β and TNF-α in serum compared with collagen peptide (P<0.01), though both of them exhibited anti-inflammation activity in aging mice. In addition, the detections of SCFAs in the intestine showed that collagen peptides had no obvious effect on the total SCFAs but increased the concentrations of acetic acid and propionic acid in the colon of aging mice, whereas TFP reversed the alterations in the concentrations of SCFAs in the colon induced by the treatment of D-galactose. Conclusion: Collagen peptides and TFP might improve the aging skin texture by increasing antioxidant activities and anti-inflammatory effects in ageing mice.