刺梨果酒对高脂诱导肥胖小鼠脂代谢的影响

陈珍; 陆敏涛; 徐方艳; 邵林子; 任廷远; 谭书明

doi:10.13386/j.issn1002-0306.2021050264

刺梨果酒对高脂诱导肥胖小鼠脂代谢的影响

Effect of Rosa roxburghii Wine on Lipid Metabolism Disorders in High Fat-induced Obese Mice

摘要

摘要: 目的：研究刺梨果酒对高脂诱导小鼠肥胖发生过程预防效果及其机理。方法：将50只小鼠随机分为空白组、模型组、刺梨果酒低剂量组（0.25 mL/80 g）、中剂量组（0.5 mL/80 g）和高剂量组（1 mL/80 g），每组10只，实验时间8周。实验结束后，测定小鼠脏器指数、血清及肝脏脂代谢相关生理生化指标；应用qRT-PCR测定肝脏过氧化物酶体增殖物激活受体α（Peroxisome proliferators-activated receptor-α, PPARα）、固醇调节元件结合蛋白（Sterol-regulatory element binding proteins, SREBP1）、硬脂酰辅酶A去饱和酶（Stearyl-coenzyme A dehydrogenase-1, SCD1）、乙酰辅酶A羧化酶（Acetyl-CoA carboxylases alpha, ACACA）、脂肪酸合成酶（Fatty acid synthase, FASN）、肝X受体（Liver X receptor, LXR）、腺苷酸活化蛋白激酶（AMP-activated protein kinase, AMPK）mRNA相对表达量。结果：与模型组相比，刺梨果酒可显著（P<0.05）减缓小鼠体重增加，降低脂肪指数及血清和肝脏中总胆固醇（Total cholesterol, TC）、甘油三酯（Triglyceride, TG）、低密度脂蛋白胆固醇（Low density lipoprotein cholesterol, LDL-C）的含量，升高高密度脂蛋白胆固醇（Hight density lipoprotein cholesterol, HDL-C）含量。此外，高剂量组可显著（P<0.05）下调小鼠肝脏中PPARα、AMPK、LXR、ACACA、SCD1、FASN基因表达水平。HE染色结果显示，剂量组能缓解肝细胞肿大，减少肝脏脂肪变性。结论：刺梨果酒对高脂诱导小鼠肥胖具有预防作用，可能与减少体内脂肪堆积，改善脂代谢紊乱有关。

Abstract: Objective: To study the preventive effect and its mechanism of Rosa roxburghii wine on obesity process in high fat-induced mice. Method: 50 mice were randomly divided into a blank group, a model group, a low-dose (0.25 mL/80 g), a medium-dose (0.5 mL/80 g) and a high-dose Rosa roxburghii wine group (1 mL/80 g), each group of 10 mice, the experiment was carried out for 8 weeks. After the experiment, the viscera coefficient, serum and liver lipid metabolism-related physiological and biochemical indicators were measured. The mRNA relative expression of PPARα (Peroxisome proliferators-activated receptor-α), SREBP1 (Sterol-regulatory element binding proteins), SCD1 (Stearyl-coenzyme A dehydrogenase-1), ACACA (Acetyl-CoA carboxylases alpha), FASN (Fatty acid synthase), LXR (Liver X receptor) and AMPK (AMP-activated protein kinase) were measured by qRT-PCR. Results: Compared with model group, Rosa roxburghii wine could significantly (P<0.05) slow down the weight gain of mice, reduce fat index and total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) content in serum and liver, and increase the content of high density lipoprotein cholesterol (HDL-C). In addition, the high-dose group significantly down-regulated the mRNA relative expressions of PPARα, AMPK, LXR, ACACA, SCD1 and FASN. The HE staining results showed that Rosa roxburghii wine could relieve hepatocellular enlargement and reduce hepatic steatosis. Conclusion: Rosa roxburghii wine has a preventive effect on high-fat-induced obesity in mice, which may be attributed to its ability to reduce fat accumulation and improve lipid metabolism disorders.