三个不同种源的铁皮石斛多糖比较及其初步的药理活性评价

黄俊彬; 丁婕; 朱海媚; 李运容; 黄丹丹; 魏刚

doi:10.13386/j.issn1002-0306.2021050262

三个不同种源的铁皮石斛多糖比较及其初步的药理活性评价

Comparison between Three Different Origins of Dendrobium officinale Polysaccharides and Their Initial Pharmacological Activity Evaluations

摘要

摘要: 本文通过对云南、丹霞、浙江三个不同种源的铁皮石斛多糖进行了同样的提取、分离、纯化，分别对其分子量、单糖组成、红外光谱进行比较，以及初步的药理活性评价，来讨论不同产地的铁皮石斛差异。采用高效凝胶渗透色谱法（High Performance Gel Permeation Chromatography，HPGPC）对纯化的多糖片段进行分子量测定、利用高效液相色谱法（High Performance Liquid Chromatography，HPLC）测定单糖组成、红外光谱（Infrared Spectroscopy, IR）测定多糖结构，应用MTT法观察多糖片段对两种肿瘤细胞（Hela细胞和HT-29细胞）增殖作用的影响。结果表明，云南种铁皮石斛多糖片段分子量为757623 u；浙江种铁皮石斛多糖片段分子量为605958 u；丹霞种铁皮石斛多糖片段分子量为663240 u。铁皮石斛多糖的单糖组成主要为甘露糖和葡萄糖，其中甘露糖与葡萄糖比值大小为云南种>丹霞种>浙江种，且多糖进行纯化后比值会进一步升高。红外光谱吸收及特征峰提示，铁皮石斛多糖主要含有甘露糖和葡萄糖，且所含糖为β型。铁皮石斛多糖纯化可以改变单糖组成比例，提高甘露糖含量，不同种源的铁皮石斛多糖在分子量、单糖组成及红外光谱吸收存在一定差异，且对HT-29肿瘤细胞具有一定的抑制作用，其中丹霞种的铁皮石斛多糖片段对两种肿瘤细胞均有较好的抑制增殖作用（48 h），具体关联性有待进一步研究。

Abstract: In this paper, comparisons of the the molecular weight, monosaccharide composition, infrared spectrum were made between Dendrobium officinale polysaccharides from three different provenances, namely Yunnan, Danxia and Zhejiang. Together with the evaluation of preliminary pharmacological activity, the differences of Dendrobium candidum from different areas were discussed. High performance gel permeation chromatography (HPGPC) was used to determine the molecular weight of the purified polysaccharide fragments, high performance liquid chromatography (HPLC) was used to determine the composition of monosaccharides, infrared spectroscopy (IR) was used to determine the structure of polysaccharides, and MTT method was used to observe the effect of polysaccharide fragments on the proliferation of two kinds of tumor cells (Hela cells and HT-29 cells). The results showed that the molecular weight of the polysaccharide fragment of Dendrobium officinale in Yunnan was 757623 u, the molecular weight of polysaccharide fragment of Dendrobium officinale in Zhejiang was 605958 u, and the molecular weight of Dendrobium officinale polysaccharide fragment in Danxia was 663240 u. The monosaccharide composition of Dendrobium officinale polysaccharide was mainly mannose and glucose. In terms of the ratio of mannose to glucose, Yunnan species>Danxia species>Zhejiang species. Moreover, the ratio of polysaccharides would further increase after purification. Infrared spectrum absorption and characteristic peaks suggested that Dendrobium officinale polysaccharides mainly contained mannose and glucose, and the sugars contained were β-type. Purification of Dendrobium officinale polysaccharides could change the composition ratio of monosaccharides and increase the content of mannose. There were certain differences in molecular weight, monosaccharide composition and infrared spectroscopy of Dendrobium officinale polysaccharides from different provenances, and they had certain inhibitory effect on HT-29 cells. Particularly, the polysaccharide fragments of Dendrobium officinale from Danxia species had a relatively good inhibitory effect on the proliferation of the two tumor cells under the 48 h experimental determination. The specific correlation needs to be further studied.