Abstract: In this study, using mint and clove as raw materials for extraction of effective substances, single factor and response surface methods were used to determine the best ratio of preservative. The results showed that when the content of mint, clove, chitosan were 2%, 3% and 1.5%, the antifungal activity against Cytospora sp. was 95.03%. During the storage of fresh-cut apples for 2~10 days, the optimal treatment group had the best preservative effect, which could delay decay rate of apples, inhibit the increase of weight loss rate, delay the degradation of ascorbic acid, reducing sugar, titratable acid and soluble solids, inhibit the growth of microbial colonies, and can better maintain the sensory quality of fresh-cut apples. The research results provided a certain reference value for the application of mint, clove and chitosan in the preservation of fresh-cut fruits.